Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/30255
Title: OXA-23-producing Acinetobacter baumannii: a new hotspot of diversity in Rio de Janeiro?
Authors: Grosso, Filipa
Carvalho, Karyne Rangel
Quinteira, Sandra
Ramos, Ana
Assef, Ana Paula D'Alincourt Carvalho
Asensi, Marise Dutra
Peixe, Luísa
Affilliation: Universidade do Porto. Faculdade de Farmácia. Laboratório de Microbiologia. REQUIMTE. Porto, Portugal.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ. Brasil.
Universidade do Porto. Faculdade de Farmácia. Laboratório de Microbiologia. REQUIMTE. Porto, Portugal / Centro de Investigação em Tecnologias da Saúde. IPSN, CESP). VN Famalicão, Portugal.
Universidade do Porto. Faculdade de Farmácia. Laboratório de Microbiologia. REQUIMTE. Porto, Portugal.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ. Brasil.
Universidade do Porto. Faculdade de Farmácia. Laboratório de Microbiologia. REQUIMTE. Porto, Portugal.
Abstract: Objectives: This study focused on the population structure of OXA-23-producing Acinetobacter baumannii clinical isolates from Rio de Janeiro, Brazil. Methods: The analysis included several genomic typing methods, including PFGE, two multilocus sequence typing (MLST) schemes, sequence group (SG) determination and blaOXA-51-like sequencing. The genomic context of the blaOXA-23 gene was also evaluated using I-CeuI hybridizations and PCR assays. Results: Congruent clustering was obtained revealing four lineages. In accordance, four new sequence types (STs) (ST131, ST132, ST133 and ST134) were obtained with the MLST-OD scheme (associated with the Oxford Database) and four (ST79, ST15 and two new allelic profiles) with the MLST-IP scheme (developed by the Institute Pasteur). Four SGs (SG1, SG4 and two new profiles) were identified, allowing the association of 70% of the isolates with European clone II. blaOXA-51-like sequencing revealed the presence of blaOXA-66, blaOXA-69, blaOXA-95 and blaOXA-132. Conclusions: Identification of new STs together with new SG profiles are findings suggestive of a local diversity hotspot that is worth exploring.
Keywords: blaOXA-23
PFGE
MLST
sequence groups
blaOXA-51-like
Acinetobacter baumannii
keywords: Acinetobacter baumannii
grupos de sequências
Issue Date: 2011
Publisher: Oxford University Press
Citation: GROSSO, Filipa; et al. OXA-23-producing Acinetobacter baumannii: a new hotspot of diversity in Rio de Janeiro?. Journal of Antimicrobial Chemotherapy, v.66, p.62-65, 2011.
DOI: 10.1093/jac/dkq406
ISSN: 0305-7453
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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