Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/30402
Title: Next-generation sequencing analyses of the emergence and maintenance of mutations in CTL epitopes in HIV controllers with differential viremia control
Authors: Caetano, Diogo Gama
Côrtes, Fernanda Heloise
Bello, Gonzalo
Teixeira, Sylvia Lopes Maia
Hoagland, Brenda
Grinsztejn, Beatriz
Veloso, Valdilea Gonçalves
Guimarães, Monick Lindenmeyer
Morgado, Mariza Gonçalves
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Aids e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Aids e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Aids e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Aids e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Aids e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Aids e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Abstract: Despite the low level of viral replication in HIV controllers (HICs), studies have reported viral mutations related to escape from cytotoxic T-lymphocyte (CTL) response in HIV-1 plasma sequences. Thus, evaluating the dynamics of the emergence of CTL-escape mutants in HICs reservoirs is important for understanding viremia control. To analyze the HIV-1 mutational profile and dynamics of CTL-escape mutants in HICs, we selected 11 long-term non-progressor individuals and divided them into the following groups: (1) viremic controllers (VCs; n = 5) and (2) elite controllers (ECs; n = 6). For each individual, we used HIV-1 proviral DNA from PBMCs related to earliest (VE) and latest (VL) visits to obtain gag and nef sequences using the Illumina HiSeq system. The consensus of each mapped gene was used to assess viral divergence, and next-generation sequencing data were employed to identify SNPs and variations within and flanking CTL epitopes.
Keywords: CTL epitope
Escape mutant
HIV controller
HIV-1
Next-generation sequencing
Single-nucleotide polymorphism
Issue Date: 2018
Publisher: BMC
Citation: CAETANO, D. G. et al. Next-generation sequencing analyses of the emergence and maintenance of mutations in CTL epitopes in HIV controllers with differential viremia control. Retrovirology, v. 15, n. 1, p. 62, 2018.
Description: Acknowledgements: We thank the patients, nurses, and clinicians who participated in the study. We also thank Egydio Sampaio for support in the recruitment of patients and all INI staff from the blood collection sector. Finally, we are thankful for the CD4+ T cell count and HIV-1 viral load clinical services from the Brazilian Ministry of Health National Network, and the FIOCRUZ PDTIS NGS Platform (RPT01J).
DOI: 10.1186/s12977-018-0444-z
ISSN: 1742-4690
Copyright: open access
Appears in Collections:INI - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Next-generation sequencing_Valdilea_Veloso_INI_Lapclin-AIDS_2018.pdf900.44 kBAdobe PDF    Request a copy


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.