Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/30411
Title: Differential apoptosis in BeWo cells after infection with highly (RH) or moderately (ME49) virulent strains of Toxoplasma gondii is related to the cytokine profile secreted, the death receptor Fas expression and phosphorylated ERK1/2 expression
Authors: Angeloni, Mariana Bodini
Guirelli, Pamela Mendonça
Franco, Priscila Silva
Barbosa, Bellisa de Freitas
Gomes, Angelica de Oliveira
Castro, Andressa da Silva
Silva, Neide Maria da
Martins Filho, Olindo Assis
Mineo, Tiago Wilson Patriarca
Silva, Deise Aparecida de Oliveira
Mineo, Jose Roberto
Ferro, Eloisa Amália Vieira
Affilliation: Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunopatologia. Uberlândia, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Doença de Chagas. Belo Horizonte, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunoparasitologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunoparasitologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunoparasitologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
Abstract: INTRODUCTION: Alterations of apoptosis are commonly associated with pregnancy complications and abortion. Modulation of apoptosis is a relevant feature of Toxoplasma gondii infection and it is related to parasite strain types. The aim of the present study was to evaluate the possible factors that are involved in the differential apoptosis of BeWo cells infected with distinct T. gondii strain types. METHODS: Human trophoblastic cells (BeWo cell line) were infected with RH or ME49 strains, the cytokine production was measured and the phosphorylation of anti-apoptotic ERK1/2 protein was analyzed. Also, cells were treated with different cytokines, infected with RH or ME49 strain, and analyzed for apoptosis index and Fas/CD95 death receptor expression. RESULTS: ME49-infected BeWo cells exhibited a predominantly pro-inflammatory cytokine profile, whereas cells infected with RH strain had a higher production of anti-inflammatory cytokines. Also, the incidence of apoptosis was higher in ME49-infected cells, which have been treated with pro-inflammatory cytokines compared to cells infected with RH and treated with anti-inflammatory cytokines. Moreover, Fas/CD95 expression was higher in cells infected with either ME49 or RH strain and treated with pro-inflammatory cytokines compared to anti-inflammatory cytokine treatment. The phosphorylation of ERK1/2 protein increased after 24 h of infection only with the RH strain. CONCLUSION: These results suggest that opposing mechanisms of interference in apoptosis of BeWo cells after infection with RH or ME49 strains of T. gondii can be associated with the differential cytokine profile secreted, the Fas/CD95 expression and the phosphorylated ERK1/2 expression.
Keywords: Apoptosis
BeWo cells
Congenital toxoplasmosis
Toxoplasma gondii
Trophoblastic cells
keywords: Apoptose
Células BeWo
Toxoplasmose congênita
Toxoplasma gondii
Células Trofoblásticas
Issue Date: 2013
Publisher: Elsevier
Citation: ANGELONI, Mariana Bodini et al. Differential apoptosis in BeWo cells after infection with highly (RH) or moderately (ME49) virulent strains of Toxoplasma gondii is related to the cytokine profile secreted, the death receptor Fas expression and phosphorylated ERK1/2 expression. Placenta, v.34, n. 11, p. 973-982, 2013
DOI: 10.1016/j.placenta.2013.09.005
ISSN: 0143-4004
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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