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MOTOR BEHAVIORAL ABNORMALITIES AND HISTOPATHOLOGICAL FINDINGS OF WISTAR RATS INOCULATED WITH HTLV-1-INFECTED MT2 CELLS
Células MT2 infectadas pelo HTLV-1
Comportamentos motores
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Abstract
The objective of the present study was to describe motor behavioral changes in association with histopathological and hematological
findings in Wistar rats inoculated intravenously with human T-cell lymphotropic virus type 1 (HTLV-1)-infected MT2
cells. Twenty-five 4-month-old male rats were inoculated with HTLV-1-infected MT2 cells and 13 control rats were inoculated
with normal human lymphocytes. The behavior of the rats was observed before and 5, 10, 15, and 20 months after inoculation
during a 30-min/rat testing time for 5 consecutive days. During each of 4 periods, a subset of rats was randomly chosen to be
sacrificed in order to harvest the spinal cord for histopathological analysis and to obtain blood for serological and molecular
studies. Behavioral analyses of the HTLV-1-inoculated rats showed a significant decrease of climbing, walking and freezing, and
an increase of scratching, sniffing, biting, licking, and resting/sleeping. Two of the 25 HTLV-1-inoculated rats (8%) developed
spastic paraparesis as a major behavioral change. The histopathological changes were few and mild, but in some cases there
was diffuse lymphocyte infiltration. The minor and major behavioral changes occurred after 10-20 months of evolution. The
long-term observation of Wistar rats inoculated with HTLV-1-infected MT2 cells showed major (spastic paraparesis) and minor
motor abnormalities in association with the degree of HTLV-1-induced myelopathy.
Keywords in Portuguese
Mielite experimentalCélulas MT2 infectadas pelo HTLV-1
Comportamentos motores
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