Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/30673
Title: Evaluation of monocyte subsets and markers of activation in leprosy reactions.
Authors: Shibuya, Mayume
Bergheme, Giovana
Passos, Sara Timoteo
Queiroz, Ivonete
Rêgo, Jamile
Carvalho, Lucas Pedreira de
Machado, Paulo R L
Affilliation: Federal University of Bahia. P os-Graduação em Ciências da Saúde. Salvador, BA, Brasil.
Federal University of Bahia. P os-Graduação em Ciências da Saúde. Salvador, BA, Brasil.
Federal University of Bahia. P os-Graduação em Ciências da Saúde. Salvador, BA, Brasil / Federal University of Bahia. Hospital Universit ario Professor Edgard Santos. Immunology Service. Salvador, BA, Brazil.
Federal University of Bahia. P os-Graduação em Ciências da Saúde. Salvador, BA, Brasil.
Federal University of Bahia. P os-Graduação em Ciências da Saúde. Salvador, BA, Brasil / Federal University of Bahia. Hospital Universit ario Professor Edgard Santos. Immunology Service. Salvador, BA, Brazil.
Federal University of Bahia. P os-Graduação em Ciências da Saúde. Salvador, BA, Brasil / Federal University of Bahia. Hospital Universit ario Professor Edgard Santos. Immunology Service. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Federal University of Bahia. P os-Graduação em Ciências da Saúde. Salvador, BA, Brasil / Federal University of Bahia. Hospital Universit ario Professor Edgard Santos. Immunology Service. Salvador, BA, Brazil.
Abstract: Understanding host immune pathways associated with tissue damage during reactions are of upmost importance to the development of immune intervention strategies. The participation of monocytes in leprosy reactions was evaluated by determining the frequency of monocyte subsets and the degree of cellular activation through the expression of MHCII and the co-stimulatory molecules CD40, CD80, CD86. Leprosy subjects with or without reactions were included in this cross-sectional study. Peripheral blood mononuclear cell were isolated and stained ex vivo to determine monocyte subsets and the degree of cellular activation by flow cytometry. Intermediate monocytes were increased in leprosy patients with reactions when compared to patients without reactions. Although no difference was detected in the frequency of monocyte subsets between type 1 and 2 reactions, the expression of CD80 was increased in monocytes from patients with type 1 reactions and CD40 was higher in paucibacillary subjects presenting type 1 reactions. Moreover, CD86 and MHC II expression were higher in intermediate monocytes when compared to the other subsets in leprosy reaction types 1 and 2. Intermediate monocyte activation with CD86 and MHCII expression is involved with both type 1 and 2 reactions, whereas CD80 and CD40 expression is related to type 1 reactions.
Keywords: Leprosy
Leprosy reactions
Monocytes
Co-stimulatory molecules
keywords: Hanseníase
Reações de lepra
Monócitos
Moléculas co-estimulatórias
Issue Date: 2018
Publisher: Elsevier
Citation: SHIBUYA, M. et al. Evaluation of monocyte subsets and markers of activation in leprosy reactions. Microbes and Infection, oct. 2018.
DOI: 10.1016/j.micinf.2018.10.003
ISSN: 1286-4579
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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