A total of 30 stool samples from 13 healthy dogs were analyzed in order to detect microorganisms in their gastrointestinal tract. The samples were enriched in alkaline peptone water added with 1% and 3% sodium chloride and Rappaport-Vassiliadis enrichment broth (37oC/18-24h). Then, they were streaked onto Pseudomonas-Aeromonas selective agar base, tiossulfate citrate bile sucrose agar, eosin methylene blue agar, and Hektoen enteric agar. After the biochemical characterization, the results showed 41 bacterial isolated strains, from which Escherichia coli, Vibrio fluvialis, and Aeromonas caviae were the main pathogens encountered.