Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/30790
Title: Effect of dexamethasone and testosterone treatment on the regulation of insulin-degrading enzyme and cellular changes in ventral rat prostate after castration
Authors: Vieira, Juliany S. B. César
Saraiva, Karina L. A.
Barbosa, Maria C. L.
Porto, Regina C. C.
Cresto, Juan C.
Peixoto, Christina A.
Wanderley, Maria I.
Udrisar, Daniel P.
Affilliation: Federal University of Pernambuco (UFPE). Department of Physiology and Pharmacology. Laboratory of Endocrinology and Metabolism. Recife, PE, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Ultraestrutura. Recife, PE, Brasil. / Center for Strategic Technologies of the Northeast (CETENE). Recife, PE, Brazil.
Federal University of Pernambuco (UFPE). Department of Physiology and Pharmacology. Laboratory of Endocrinology and Metabolism. Recife, PE, Brazil.
Federal University of Pernambuco (UFPE). Department of Physiology and Pharmacology. Laboratory of Endocrinology and Metabolism. Recife, PE, Brazil.
Hospital de Niños Ricardo Gutièrrez. Department of Endocrinology (CEDIE). Buenos Aires, Argentina.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Ultraestrutura. Recife, PE, Brasil. / Center for Strategic Technologies of the Northeast (CETENE). Recife, PE, Brazil.
Federal University of Pernambuco (UFPE). Department of Physiology and Pharmacology. Laboratory of Endocrinology and Metabolism. Recife, PE, Brazil.
Federal University of Pernambuco (UFPE). Department of Physiology and Pharmacology. Laboratory of Endocrinology and Metabolism. Recife, PE, Brazil.
Abstract: Insulin-degrading enzyme (IDE) has been shown to enhance the binding of androgen and glucocorticoid receptors to DNA in the nuclear compartment. Glucocorticoids cause hyperglycaemia, peripheral resistance to insulin and compensatory hyperinsulinaemia. The aim of the present study was to investigate the effect of dexamethasone (D), testosterone (T) and dexamethasone plus testosterone (D + T) on the regulation of IDE and on the remodelling of rat ventral prostate after castration (C). Castration led to a marked reduction in prostate weight (PW). Body weight was significantly decreased in the castrated animals treated with dexamethasone, and the relative PW was 2.6-fold (±0.2) higher in the D group, 2.8-fold (±0.3) higher in the T group and 6.6-fold (±0.6) higher in the D + T group in comparison with the castrated rats. Ultrastructural alterations in the ventral prostate in response to androgen deprivation were restored after testosterone and dexamethasone plus testosterone treatments and partially restored with dexamethasone alone. The nuclear IDE protein level indicated a 4.3-fold (±0.4) increase in castrated rats treated with D + T when compared with castration alone. Whole-cell IDE protein levels increased approximately 1.5-fold (±0.1), 1.5-fold (±0.1) and 2.9-fold (±0.2) in the D, T and D + T groups, respectively, when compared with castration alone. In conclusion, the present study reports that dexamethasone-induced hyperinsulinaemic condition plus exogenous testosterone treatment leads to synergistic effects of insulin and testosterone in the prostatic growth and in the amount of IDE in the nucleus and whole epithelial cell.
Keywords: Castration
Glucocorticoid
Insulin resistance
Insulin-degrading enzyme
Prostate
Testosterone
Ultrastructure
DeCS: Andrógenos / farmacologia
Animais
Peso Corporal / efeitos de drogas
Castração
Núcleo Celular / efeitos de drogas
Núcleo Celular / metabolismo
Núcleo Celular / Patologia
Dexametasona / efeitos adversos
Dexametasona / farmacologia
Glucocorticóides / efeitos adversos
Glucocorticóides / farmacologia
Hiperinsulinismo / induzido quimicamente
Hiperinsulinismo / metabolismo
Insulysin / efeitos de drogas
Insulisina / metabolismo
Masculino
Modelos Animal
Próstata / efeitos de drogas
Próstata / metabolismo
Próstata / patologia
Ratos
Ratos Wistar
Testosterona / farmacologia
Issue Date: 2011
Citation: VIEIRA, Juliany S. B. César et al. Effect of Dexamethasone and Testosterone Treatment on the Regulation of Insulin-Degrading Enzyme and Cellular Changes in Ventral Rat Prostate after Castration. International Journal of Experimental Pathology, v. 92, n. 4, p. 272–280, ago. 2011.
DOI: 10.1111/j.1365-2613.2011.00772.x
ISSN: 1365-2613
Copyright: restricted access
Appears in Collections:PE - IAM - Artigos de Periódicos

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