Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/30813
Title: Trypanosoma cruzi in Brazilian Amazonia: Lineages TCI and TCIIa in wild primates, Rhodnius spp. and in humans with Chagas disease associated with oral transmission
Authors: Marcili, Arlei
Valente, Vera C.
Valente, Sebastião A.
Junqueira, Angela C. V.
Silva, Flávia Maia da
Pinto, Ana Yecê das Neves
Naiff, Roberto D.
Campaner, Marta
Coura, José Rodrigues
Camargo, Erney P.
Miles, Michael A.
Teixeira, Marta M. G.
Affilliation: Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, Brasil.
Instituto Evandro Chagas. Laboratório de Doença de Chagas. Belém, PA, Brasil.
Instituto Evandro Chagas. Laboratório de Doença de Chagas. Belém, PA, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil.
Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, Brasil.
Instituto Evandro Chagas. Laboratório de Doença de Chagas. Belém, PA, Brasil.
Instituto Nacional de Pesquisas das Amazônia. Manaus, AM, Brasil
Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil.
Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, Brasil.
London School of Hygiene and Tropical Medicine. Department of Infectious an Tropical Diseases. London, UK.
Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, Brasil.
Abstract: In this study, we provide phylogenetic and biogeographic evidence that the Trypanosoma cruzi lineages T. cruzi I (TCI) and T. cruzi IIa (TCIIa) circulate amongst non-human primates in Brazilian Amazonia, and are transmitted by Rhodnius species in overlapping arboreal transmission cycles, sporadically infecting humans. TCI presented higher prevalence rates, and no lineages other than TCI and TCIIa were found in this study in wild monkeys and Rhodnius from the Amazonian region. We characterised TCI and TCIIa from wild primates (16 TCI and five TCIIa), Rhodnius spp. (13 TCI and nine TCIIa), and humans with Chagas disease associated with oral transmission (14 TCI and five TCIIa) in Brazilian Amazonia. To our knowledge, TCIIa had not been associated with wild monkeys until now. Polymorphisms of ssrDNA, cytochrome b gene sequences and randomly amplified polymorphic DNA (RAPD) patterns clearly separated TCIIa from TCIIb-e and TCI lineages, and disclosed small intra-lineage polymorphisms amongst isolates from Amazonia. These data are important in understanding the complexity of the transmission cycles, genetic structure, and evolutionary history of T. cruzi populations circulating in Amazonia, and they contribute to both the unravelling of human infection routes and the pathological peculiarities of Chagas disease in this region.
Keywords: Trypanosoma cruzi lineages TCI and TCIIa
Trypanosoma cruzi
oral infection
Non-Human primates
Amazonia
ssrDNA
Cythocrome b
Evolution
Phylogeny
keywords: Trypanosoma cruzi
Doença de Chagas
Infecção oral
Primates não-humanos
Amazônia
Evolução
Filogenia
Citocromo b
Issue Date: 2009
Publisher: Elsevier
Citation: MARCILI, Arlei; et al. Trypanosoma cruzi in Brazilian Amazonia: Lineages TCI and TCIIa in wild primates, Rhodnius spp. in human with Chagas Disease associated with oral transmission. International Journal for Pathology v.39, p.615-623, 2009.
DOI: 10.1016/j.ijpara.2008.09.015
ISSN: 0020-7519
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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