| Author | Silva, Marialice da Fonseca Ferreira da | |
| Author | Takács, Anna C. | |
| Author | Barbosa, Helene S. | |
| Author | Gross, Uwe | |
| Author | Lüder, Carsten G. K. | |
| Access date | 2019-01-03T12:05:12Z | |
| Available date | 2019-01-03T12:05:12Z | |
| Document date | 2009 | |
| Citation | SILVA, Marialice da Fonseca Ferreira da; et al. Primary skeletal muscle cells trigger spontaneous Toxoplasma gondii tachyzoite-to-bradyzoite conversion at higher rates than fibroblasts. International Journal of Medical Microbiology, v.299, p.381-388, 2009. | pt_BR |
| ISSN | 1438-4221 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/30846 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Toxoplasma gondii | pt_BR |
| Subject in Portuguese | Persistência do parasita | pt_BR |
| Subject in Portuguese | Células musculares esqueléticas | pt_BR |
| Subject in Portuguese | Conversão espontânea | pt_BR |
| Subject in Portuguese | taquizoítos | pt_BR |
| Subject in Portuguese | bradizitos | pt_BR |
| Subject in Portuguese | fibroblastos | pt_BR |
| Title | Primary skeletal muscle cells trigger spontaneous Toxoplasma gondii tachyzoite-to-bradyzoite conversion at higher rates than fibroblasts | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.ijmm.2008.10.002 | |
| Abstract | Toxoplasma gondii is one of the most common eukaryotic parasites and an important opportunistic pathogen of humans. The interconversion from proliferative tachyzoites into quiescent encysted bradyzoites plays pivotal roles in the lifelong persistence of T. gondii in its host and the pathogenesis of toxoplasmosis. Stage conversion and persistence in skeletal muscle tissue may be particularly important for the food-borne transmission of T. gondii to humans via raw or undercooked meat products. Here, we have followed the transition of tachyzoites to bradyzoites after infection of skeletal muscle cells (SkMC). Primary murine myoblasts were differentiated to multinucleated syncytial myotubes that displayed regular contractions in vitro and expressed myogenic markers Myf5 and MyoD. Tachyzoites of T. gondii invaded SkMC within 4h of infection and started to replicate within 24h of infection. Remarkably, intracellular tachyzoites readily differentiated to bradyzoites in SkMC without the need of exogenous stress factors. Double immunofluorescence labelling revealed significantly higher percentages of bradyzoite-containing vacuoles in SkMC than in murine fibroblasts at 24h until 6 days after infection. Furthermore, transcript levels of bradyzoite-specific ENO1 but not tachyzoite-specific ENO2 strongly increased in T. gondii-infected SkMC until 6 days of infection. These findings indicate that the commitment of T. gondii to differentiate to bradyzoites in SkMC does not require exogenous stress factors but could be rather regulated by cell-type specific factors. | pt_BR |
| Affilliation | Georg-August-University. Institute for Medical Microbiology. Göttingen, Germany / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Georg-August-University. Institute for Medical Microbiology. Göttingen, Germany. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Georg-August-University. Institute for Medical Microbiology. Göttingen, Germany. | pt_BR |
| Affilliation | Georg-August-University. Institute for Medical Microbiology. Göttingen, Germany. | pt_BR |
| Subject | Toxoplasma gondii | pt_BR |
| Subject | Skeletal muscle cell | pt_BR |
| Subject | Stage conversion | pt_BR |
| Subject | Bradyzoite | pt_BR |
| Subject | Parasite persistence | pt_BR |
| Subject | tachyzoite | pt_BR |
| Subject | fibroblasts | pt_BR |
| e-ISSN | 1618-0607 | |
| Embargo date | 2030-01-01 | |
