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IN HOUSE COLORIMETRIC REVERSE HYBRIDISATION ASSAY FOR DETECTION OF THE MUTATION MOST FREQUENTLY ASSOCIATED WITH RESISTANCE TO ISONIAZID IN MYCOBACTERIUM TUBERCULOSIS
Resistência a medicamentos
ensaio de hibridização reversa
Isoniazida
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Abstract
Mutations in the katG gene have been identified and correlated with isoniazid (INH) resistance in Mycobacterium
tuberculosis isolates. The mutation AGC→ACC (Ser→Thr) at katG315 has been reported to be the most frequent
and is associated with transmission and multidrug resistance. Rapid detection of this mutation could therefore
improve the choice of an adequate anti-tuberculosis regimen, the epidemiological monitoring of INH resistance and,
possibly, the tracking of transmission of resistant strains. An in house reverse hybridisation assay was designed in
our laboratory and evaluated with 180 isolates of M. tuberculosis. It could successfully characterise the katG315
mutation in 100% of the samples as compared to DNA sequencing. The test is efficient and is a promising alternative
for the rapid identification of INH resistance in regions with a high prevalence of katG315 mutants.
Keywords in Portuguese
TuberculoseResistência a medicamentos
ensaio de hibridização reversa
Isoniazida
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