Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/30953
Title: Effects of low-protein diet on Schistosoma mansoni morphology visualized by morphometry and confocal laser scanning microscopy
Authors: Barros, L. A.
Neves, R. H.
Moura, E. G. de
Silva, J. R. Machado
Affilliation: Universidade do Estado do Rio de Janeiro. Centro Biomédico. Faculdade de Ciências Medicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Helmintologia Romero Lascasas Porto. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Helmintos Parasitos de Vertebrados. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Centro Biomédico. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Ciências Fisiológicas. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Centro Biomédico. Faculdade de Ciências Medicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Helmintologia Romero Lascasas Porto. Rio de Janeiro, RJ, Brasil.
Abstract: Previous studies have shown that protein deficiencies can hamper both the course of experimental schistosomiasis and normal development of adult worms. To further investigate this relationship, we compared adult male and female Schistosoma mansoni from malnourished and well-fed mice through morphometric and confocal laser scanning microscopy analysis. Swiss mice were fed protein-deficient diets (8%) and infected subcutaneously with approximately 80 S. mansoni cercariae (BH strain, Brazil). Control mice were fed a standard rodent diet (23% protein). The nutritional status was evaluated by body weight gain and albumin values. Mice were sacrificed 63 days post-infection. Recovered worms were stained with hydrochloric carmine and preserved as whole-mounts for bright-field examination and confocal microscopy. The body weight gain and serum albumin concentrations were significantly lower (P < 0.05) in malnourished mice than in controls. In general, all morphometric values of specimens grown in malnourished mice were lower than those of control mice. Schistosome worms grown in malnourished mice had statistically significant differences (P < 0.05) in the reproductive system and tegument than those grown in mice fed standard diets. In female worms, vitelline glands showed few remaining follicles and ovaries lacked mature oocytes. In male parasites, tubercles were fewer in number on the dorsal surface and testicular lobes presented fewer differentiated germinal cells. In summary, we describe novel data supporting the view that low-protein diets may influence the development of adult worms.
Keywords: Schistosoma mansoni
Morphology
Confocal laser scanning microscopy
Low-protein diet
keywords: Schistosoma mansoni
Morfologia
Microscopia confocal de varredura a laser
Dieta baixa em proteínas
Issue Date: 2009
Publisher: Cambridge University Press
Citation: BARROS, L. A. et al. Effects of low-protein diet on Schistosoma mansoni morphology visualized by morphometry and confocal laser scanning microscopy. Journal of Helminthology, v. 83, p.13-21, 2009.
DOI: 10.1017/S0022149X08067217
ISSN: 0022-149X
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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