| Author | Almeida-Silva, Fernando | |
| Author | Barbedo, Leonardo Silva | |
| Author | Taylor, Maria Lucia | |
| Author | Muniz, Mauro de Medeiros | |
| Author | Guimarães, Allan Jefferson | |
| Author | Zancopé-Oliveira, Rosely Maria | |
| Access date | 2019-01-10T13:36:37Z | |
| Available date | 2019-01-10T13:36:37Z | |
| Document date | 2018 | |
| Citation | ALMEIDA-SILVA, Fernanda et al. Multiplex polymerase chain reaction as an improved method for screening Histoplasma capsulatum mating types. Memórias do Instiuto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 10, p. 1-4, 2018. | pt_BR |
| ISSN | 1678-8060 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/30959 | |
| Language | eng | pt_BR |
| Publisher | Fiocruz/IOC | pt_BR |
| Rights | open access | pt_BR |
| Title | Multiplex polymerase chain reaction as an improved method for screening Histoplasma capsulatum mating types | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1590/0074-02760180340 | |
| Abstract | Histoplasmosis is a systemic mycosis infection caused by Histoplasma capsulatum, a heterothallic ascomycete. The sexual reproduction of this fungus is regulated by the mating type (MAT1) locus that contains MAT1-1 and MAT1-2 idiomorphs, which were identified by uniplex polymerase chain reaction (PCR). This study aimed to optimise single-step multiplex PCR for the accurate detection of the distinct mating types of H. capsulatum. Among the 26 isolates tested, 20 had MAT1-1 genotype, while six showed MAT1-2 genotype, in agreement with the uniplex PCR results. These results suggest that multiplex PCR is a fast and specific tool for screening H. capsulatum mating types. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil / Universidade Federal do Amazonas. Instituto de Saúde e Biotecnologia. Coari, AM, Brasil. | pt_BR |
| Affilliation | Universidad Nacional Autónoma de México. Facultad de Medicina. Departamento de Microbiología-Parasitología. DF, México. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Mating type | pt_BR |
| Subject | MAT1 | pt_BR |
| Subject | Histoplasma capsulatum | pt_BR |
| Subject | Multiplex PCR | pt_BR |
