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MULTIPLEX POLYMERASE CHAIN REACTION AS AN IMPROVED METHOD FOR SCREENING HISTOPLASMA CAPSULATUM MATING TYPES
Almeida-Silva, Fernando et al. | Date Issued: 2018
Author
Almeida-Silva, Fernando
Barbedo, Leonardo Silva
Taylor, Maria Lucia
Muniz, Mauro de Medeiros
Guimarães, Allan Jefferson
Zancopé-Oliveira, Rosely Maria
Affilliation
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil / Universidade Federal do Amazonas. Instituto de Saúde e Biotecnologia. Coari, AM, Brasil.
Universidad Nacional Autónoma de México. Facultad de Medicina. Departamento de Microbiología-Parasitología. DF, México.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil.
Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil.
Abstract
Histoplasmosis is a systemic mycosis infection caused by Histoplasma capsulatum, a heterothallic ascomycete. The sexual reproduction of this fungus is regulated by the mating type (MAT1) locus that contains MAT1-1 and MAT1-2 idiomorphs, which were identified by uniplex polymerase chain reaction (PCR). This study aimed to optimise single-step multiplex PCR for the accurate detection of the distinct mating types of H. capsulatum. Among the 26 isolates tested, 20 had MAT1-1 genotype, while six showed MAT1-2 genotype, in agreement with the uniplex PCR results. These results suggest that multiplex PCR is a fast and specific tool for screening H. capsulatum mating types.
Keywords
Mating type
MAT1
Histoplasma capsulatum
Multiplex PCR
 
Publisher
Fiocruz/IOC
Citation
ALMEIDA-SILVA, Fernanda et al. Multiplex polymerase chain reaction as an improved method for screening Histoplasma capsulatum mating types. Memórias do Instiuto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 10, p. 1-4, 2018.
DOI
10.1590/0074-02760180340
ISSN
1678-8060