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INHIBITION OF TRYPANOSOMA CRUZI PROLINE RACEMASE AFFECTS HOST-PARASITE INTERACTIONS AND THE OUTCOME OF IN VITRO INFECTION
Coutinho, Leticia et al. | Date Issued: 2009
Author
Coutinho, Leticia
Ferreira, Marcelo Alves
Cosson, Alain
Batista, Marcos Meuser
Batista, Denise da Gama Jaén
Minoprio, Paola
Degrave, Win M.
Berneman, Armand
Soeiro, Maria Nazaré C.
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.
Institut Pasteur. Laboratoire d’Immunobiologie des infections à Trypanosoma. Département d’Immunologie. Paris, France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Institut Pasteur. Laboratoire d’Immunobiologie des infections à Trypanosoma. Département d’Immunologie. Paris, France.
undação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.
Institut Pasteur. Laboratoire d’Immunobiologie des infections à Trypanosoma. Département d’Immunologie. Paris, France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Abstract
Proline racemase is an important enzyme of Trypanosoma cruzi and has been shown to be an effective mitogen for B cells, thus contributing to the parasite’s immune evasion and persistence in the human host. Recombinant epimastigote parasites overexpressing TcPRAC genes coding for proline racemase present an augmented ability to differentiate into metacyclic infective forms and subsequently penetrate host-cells in vitro. Here we demonstrate that both anti T. cruzi proline racemase antibodies and the specific proline racemase inhibitor pyrrole-2-carboxylic acid significantly affect parasite infection of Vero cells in vitro. This inhibitor also hampers T. cruzi intracellular differentiation.
Keywords in Portuguese
Trypanosoma cruzi
Prolina racemase
Inibição enzimática
Interação parasita-hospedeiro
 
Keywords
Trypanosoma cruzi
Host-parasite interaction
Enzyme inhibition
Proline racemase
 
Publisher
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
Citation
COUTINHO, Leticia et al. Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n. 8, p. 1055-1062, Dec. 2009.
ISSN
0074-0276