| Author | Soares, Afonso J. C. | |
| Author | Santos, M. F. | |
| Author | Trugilho, M. R. O. | |
| Author | Neves-Ferreira, A. G. C. | |
| Author | Perales, Jonas | |
| Author | Domont, G. B. | |
| Access date | 2019-01-10T15:36:37Z | |
| Available date | 2019-01-10T15:36:37Z | |
| Document date | 2009 | |
| Citation | SOARES, Afonso J. C. et al. Differential proteomics of the plasma of individuals with sepsis caused by Acinetobacter baumannii. Journal of Proteomics, v. 73, p. 267-278, 2009. | pt_BR |
| ISSN | 1874-3919 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/30970 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Acinetobacter baumannii | pt_BR |
| Subject in Portuguese | Proteoma diferencial | pt_BR |
| Subject in Portuguese | Sepse | pt_BR |
| Subject in Portuguese | Depleção de proteína plasmática | pt_BR |
| Subject in Portuguese | Técnica DIGE | pt_BR |
| Title | Differential proteomics of the plasma of individuals with sepsis caused by Acinetobacter baumannii | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.jprot.2009.09.010 | |
| Abstract | This study examines alterations in the plasma proteome in ten adults affected by sepsis caused by Acinetobacter baumannii as compared to paired healthy controls. 2-DE profiles of plasma from patients and paired healthy donors, depleted of the six most abundant proteins, were analysed by the DIGE technique. Protein spot detection and quantification were performed with the Differential In-gel Analysis and Biological Variation Analysis modules of the DeCyder() software. Differentially expressed proteins were identified by mass spectrometry (MALDI-TOF/TOF) after colloidal Coomassie blue staining. Almost 900 spots were detected on a unique 2-D gel by the DIGE technique. A total of 269 protein spots of differential abundance were shown to be statistically significant (2.5-fold) with p values of p< or =0.01 (135 spots) and p< or =0.05 (134 spots) as determined by the t test. Seventy-one spots were submitted to mass spectrometry and about 30% could be successfully identified. This multiplex approach significantly reduced experimental variability, allowing for the confident detection of small differences in protein levels. Results include differentially expressed lipoproteins as well as proteins belonging to inflammatory/coagulation pathways and the kallikrein-kinin system. These data improves the knowledge for future developments in sepsis diagnosis, staging and therapy. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química.Unidade de Proteômica. Laboratório de Química Proteica. Rio de Janeiro, RJ, Brasil / Hospital da Força Aérea Brasileira. Rio de Janeiro, RJ, Brasil / Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química.Unidade de Proteômica. Laboratório de Química Proteica. Rio de Janeiro, RJ, Brasil / Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química.Unidade de Proteômica. Laboratório de Química Proteica. Ri de Janeiro, RJ, Brasil / Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Sepsis | pt_BR |
| Subject | Acinetobacter baumannii | pt_BR |
| Subject | Plasma protein depletion | pt_BR |
| Subject | DIGE | pt_BR |
| Subject | Differential proteome | pt_BR |
| Embargo date | 2030-01-01 | |
