| Author | Cunha, José Maurício Albuquerque | |
| Author | Gonzalez, Marcelo Salabert | |
| Author | Garcia, Eloi de Souza | |
| Author | Mello Neto, Cícero Brasileiro de | |
| Author | Azambuja, Patricia de | |
| Author | Almeida, João Carlos de Aquino | |
| Author | Souza, Wanderley de | |
| Author | Nogueira, Nadir Francisca Sant'Anna | |
| Access date | 2019-01-10T16:54:05Z | |
| Available date | 2019-01-10T16:54:05Z | |
| Document date | 2009 | |
| Citation | CUNHA, José Maurício Albuquerque et al. Cytochemical characterization of microvillar and perimicrovillar membranes in the posterior midgut epithelium of Rhodnius prolixus. Arthropod Structure & Development, v. 38, n. 1, p. 31-44, Jan. 2009. | |
| ISSN | 1467-8039 | |
| URI | https://www.arca.fiocruz.br/handle/icict/30979 | |
| Description | Produção científica do Laboratório de Bioquímica e Fisiologia de Insetos. | pt_BR |
| Language | eng | en_US |
| Publisher | Elsevier | |
| Rights | restricted access | |
| Subject in Portuguese | Rhodnius prolixus | pt_BR |
| Subject in Portuguese | Moléculas de ligação a carboidratos | pt_BR |
| Subject in Portuguese | Citoquímica | pt_BR |
| Subject in Portuguese | Membranas perimicrovilares e peritróficas | pt_BR |
| Subject in Portuguese | Matriz | pt_BR |
| Title | Cytochemical characterization of microvillar and perimicrovillar membranes in the posterior midgut epithelium of Rhodnius prolixus | en_US |
| Type | Article | |
| DOI | 10.1016/j.asd.2008.06.001 | |
| Abstract | Perimicrovillar membranes (PMM) are structures present on the surface of midgut epithelial cells of the hematophagous insect, Rhodnius prolixus. They cover the microvilli and are especially evident 10 days after blood meal, providing the compartmentalization of the enzymatic processes in the intestinal microenvironment. Using an enzyme cytochemical approach, Mg2+-ATPase and ouabain-sensitive Na+K+-ATPase activities were observed in the plasma (or microvillar) membrane (MM) of midgut cells and in the PMM. In contrast, alkaline phosphatase was only detected in MM. Using cationized ferritin and colloidal iron hydroxide particles, anionic sites were found only on the luminal surface of the PMM. Using fluorescein isothiocyanate (FITC)-labeled lectins, residues of alpha-d-galactose, mannose, N-acetyl-neuraminic acid, N-acetyl-d-galactosamine and N-acetyl-galactosamine-alpha-1,3-galactose were detected on the apical surface of posterior midgut epithelial cells. On the other hand, using FITC-labeled neoglycoproteins (NGP) it was possible to detect the presence of carbohydrate binding molecules (CBM) recognizing N-acetyl-d-galactosamine, alpha-d-mannose, alpha-l-fucose and alpha-d-glucose in the posterior midgut epithelium. The use of digitonin showed the presence of sterols in the MM and PMM. These results have led the authors to suggest that for some components the PMM resembles the MM lining the midgut cells of R. prolixus, composing a system which covers the microvilli and stretches to the luminal space. | en_US |
| Affilliation | Universidade Estadual do Norte Fluminense. Centro de Biociências e Biotecnologia. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacazes, RJ, Brasil. | |
| Affilliation | Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Estadual do Norte Fluminense. Centro de Biociências e Biotecnologia. Laboratório de Fisiologia e Bioquímica de Microrganismos. Campos dos Goytacazes, RJ, Brasil. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Estadual do Norte Fluminense. Centro de Biociências e Biotecnologia. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacazes, RJ, Brasil. | |
| Subject | Rhodnius prolixus | en_US |
| Subject | Histocitoquímica | en_US |
| Subject | Perimicrovillar membranes and peritrophic | en_US |
| Subject | Matrix | en_US |
| Subject | Surface anionic sites | en_US |
| Subject | Carbohydrate-binding molecules | en_US |
| DeCS | Histocitoquímica | pt_BR |
