Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/30979
Title: Cytochemical characterization of microvillar and perimicrovillar membranes in the posterior midgut epithelium of Rhodnius prolixus
Authors: Cunha, José M. Albuquerque
Gonzalez, Marcelo S.
Garcia, Elói S.
Mello, Cícero B.
Azambuja, Patrícia
Almeida, João C. A.
Souza, Wanderley de
Nogueira, Nadir F. S.
Affilliation: Universidade Estadual do Norte Fluminense. Centro de Biociências e Biotecnologia. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacazes, RJ, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Bioquímica de Insetos. Rio de Janeiro, RJ, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Bioquímica de Insetos. Rio de Janeiro, RJ, Brasil.
Universidade Estadual do Norte Fluminense. Centro de Biociências e Biotecnologia. Laboratório de Fisiologia e Bioquímica de Microrganismos. Campos dos Goytacazes, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil.
Universidade Estadual do Norte Fluminense. Centro de Biociências e Biotecnologia. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacazes, RJ, Brasil.
Abstract: Perimicrovillar membranes (PMM) are structures present on the surface of midgut epithelial cells of the hematophagous insect, Rhodnius prolixus. They cover the microvilli and are especially evident 10 days after blood meal, providing the compartmentalization of the enzymatic processes in the intestinal microenvironment. Using an enzyme cytochemical approach, Mg2+-ATPase and ouabain-sensitive Na+K+-ATPase activities were observed in the plasma (or microvillar) membrane (MM) of midgut cells and in the PMM. In contrast, alkaline phosphatase was only detected in MM. Using cationized ferritin and colloidal iron hydroxide particles, anionic sites were found only on the luminal surface of the PMM. Using fluorescein isothiocyanate (FITC)-labeled lectins, residues of alpha-d-galactose, mannose, N-acetyl-neuraminic acid, N-acetyl-d-galactosamine and N-acetyl-galactosamine-alpha-1,3-galactose were detected on the apical surface of posterior midgut epithelial cells. On the other hand, using FITC-labeled neoglycoproteins (NGP) it was possible to detect the presence of carbohydrate binding molecules (CBM) recognizing N-acetyl-d-galactosamine, alpha-d-mannose, alpha-l-fucose and alpha-d-glucose in the posterior midgut epithelium. The use of digitonin showed the presence of sterols in the MM and PMM. These results have led the authors to suggest that for some components the PMM resembles the MM lining the midgut cells of R. prolixus, composing a system which covers the microvilli and stretches to the luminal space.
Keywords: Rhodnius prolixus
Histocitoquímica
Perimicrovillar membranes and peritrophic
Matrix
Surface anionic sites
Carbohydrate-binding molecules
keywords: Rhodnius prolixus
Moléculas de ligação a carboidratos
Citoquímica
Membranas perimicrovilares e peritróficas
Matriz
DeCS: Histocitoquímica
Issue Date: 2009
Publisher: Elsevier
Citation: CUNHA, José M. Albuquerque et al. Cytochemical characterization of microvillar and perimicrovillar membranes in the posterior midgut epithelium of Rhodnius prolixus. Arthropod Structure & Development, v. 38, p. 31-44, 2009.
DOI: 10.1016/j.asd.2008.06.001
ISSN: 1467-8039
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
patriazambuja_eloigarcia_etal_IOC_2009.pdf2.88 MBAdobe PDF    Request a copy


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.