Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31087
Title: Detection of virulence-associated and regulatory protein genes in association with phage typing of human Vibrio cholerae from several geographical regions of the world
Authors: Bhowmick, Tushar Suvra
Das, Mayukh
Ruppitsch, Werner
Stoeger, Anna
Pietzka, Tatjana
Allerberger, Franz
Rodrigues, Dália P.
Sarker, B. L.
Affilliation: National Institute of Cholera and Enteric Diseases. Division of Bacteriology. Kolkata, India.
National Institute of Cholera and Enteric Diseases. Division of Bacteriology. Kolkata, India.
Austrian Agency for Health and Food Safety. Vienna, Austria.
Austrian Agency for Health and Food Safety. Vienna, Austria.
Austrian Agency for Health and Food Safety. Vienna, Austria.
Austrian Agency for Health and Food Safety. Vienna, Austria.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
National Institute of Cholera and Enteric Diseases. Division of Bacteriology. Kolkata, India.
Abstract: Vibrio cholerae O1, O139 and occasionally non-O1/non-O139 serogroups are most often responsible for epidemic and pandemic cholera. This study used genotypic patterns of PCRbased detection of virulence-associated and regulatory protein genes, along with phage typing, to characterize 86 V. cholerae strains. Thirty-eight of 53 O1 biotype El Tor strains harboured both tcpA classical and tcpA El Tor genes, and three El Tor strains lacked the V. cholerae O1-specific gene (Vc-O1); three O139 strains contained both Vc-O1 and Vc-O139 genes and seven out of ten non-O1/non-O139 strains possessed the Vc-O1 gene. The latter strains all harboured the virulence-associated genes ctxA, zot, ace, RS1, hlyA, ompU, rtxA and sxt. Two phage types, T27 and T25, were predominant in strains from different geographical regions of India, whereas more variation in phage susceptibility was observed for tetracycline-resistant strains from Kolkata. These results suggest that the pattern and distribution of virulence genes and phage types of V. cholerae are equally useful and discriminatory in tracing the origin of newly emerging strains.
Keywords: Human Vibrio cholerae
virulence
regulatory protein genes
phage typing
keywords: Vibrio cholerae humano
virulência
genes de proteínas
tipagem fágica
Issue Date: 2009
Publisher: Microbiology Society
Citation: BHOWMICK, Tushar Suvra; et al. Detection of virulence-associated and regulatory protein genes in association with phage typing of human Vibrio cholerae from several geographical regions of the world. Journal of Medical Microbiology, v.58, p.1160–1167, 2009.
DOI: 10.1099/jmm.0.008466-0
ISSN: 0022-2615
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
DP_Rodrigues_etal_IOC_2009.pdf452.23 kBAdobe PDF    Request a copy


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.