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2030-01-01
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DEVELOPMENT OF SCHISTOSOMA MANSONI IN THE LABORATORY RAT ANALYZED BY LIGHT AND CONFOCAL LASER SCANNING MICROSCOPY
Leitão, F. W. Silva et al. | Date Issued: 2009
Author
Leitão, F. W. Silva
Biolchini, C. L.
Neves, R .H.
Silva, J. R. Machado
Affilliation
Universidade do Estado do Rio de Janeiro. Centro Biomédico. Faculdade de Ciências Medicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Helmintologia Romero Lascasas Porto. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Centro Biomédico. Faculdade de Ciências Medicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Helmintologia Romero Lascasas Porto. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Helmintos Parasitos de Vertebrados. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Centro Biomédico. Faculdade de Ciências Medicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Helmintologia Romero Lascasas Porto. Rio de Janeiro, RJ, Brasil.
Abstract
The kinetic of maturation (schistogram) of Schistosoma mansoni worms grown in laboratory rats was studied by light and confocal laser scanning microscopy. Infected rats with the BH strain were weekly euthanized 3-9weeks pi. Recovered flukes stained with hydrochloric carmine were preserved as whole-mounts and analyzed by confocal and brightfield microscopy. Worms displayed varying degrees of maturation of the reproductive system at weeks 3-6. Male worms showed complete maturation of the reproductive system at week 6, while female worms completed their maturation at week 7. Males presented few tubercles in tegument in all weeks. Despite the presence of a developing embryo within the ootype, no uterine egg was found. The schistogram in rats follows a pattern similar to that observed in mice hosts.
Keywords in Portuguese
Schistosoma mansoni
Rattus norvegicus
Rato branco
Microscopia Confocal
cinética da maturação
 
Keywords
Schistosoma mansoni
Rattus norvegicus
Confocal microscopy
White rat
Schistogram
 
Publisher
Elsevier
Citation
LEITÃO, F. W. Silva et al. Development of Schistosoma mansoni in the laboratory rat analyzed by light and confocal laser scanning microscopy. Experimental Parasitology, v. 123, p. 292–295, 2009.
DOI
10.1016/j.exppara.2009.07.016
ISSN
0014-4894