Author | Echevarria-Lima, Juliana | |
Author | Pereira, Denise de Abreu | |
Author | Oliveira, Thais Silva de | |
Author | Espíndola, Otávio de Melo | |
Author | Lima, Marco Antonio | |
Author | Leite, Ana Cláudia Celestino | |
Author | Sandim, Vanessa | |
Author | Nascimento, Clarissa Rodrigues | |
Author | Kalume, Dario E. | |
Author | Zingali, Russolina B. | |
Access date | 2019-01-17T22:30:44Z | |
Available date | 2019-01-17T22:30:44Z | |
Document date | 2018 | |
Citation | ECHEVARRIA-LIMA, Juliana et al. Protein profile of blood monocytes is altered in HTLV-1 infected patients: implications for HAM/TSP disease. Scientific Reports, v. 8, p. 1-14, Sept. 2018. | pt_BR |
ISSN | 2045-2322 | |
URI | https://www.arca.fiocruz.br/handle/icict/31134 | |
Language | eng | pt_BR |
Publisher | Nature Research | pt_BR |
Rights | open access | |
Title | Protein profile of blood monocytes is altered in HTLV-1 infected patients: implications for HAM/TSP disease | pt_BR |
Type | Article | pt_BR |
DOI | 10.1038/s41598-018-32324-2 | |
Abstract | Human T-cell lymphotropic virus type-1 (HTLV-1) is the etiological agent of HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). The endothelial breakdown and migration of leukocytes, including monocytes, to the spinal cord are involved in HAM/TSP development. Monocytes from HTLV-1-infected individuals exhibit important functional diferences when compared to cells from uninfected donors. Using proteomic shot gun strategy, performed by nanoACQUITY-UPLC system, we analyzed monocytes isolated from peripheral blood of asymptomatic carriers (AC), HAM/TSP and uninfected individuals. 534 proteins were identifed among which 376 were quantifed by ExpressionE software. Our study revealed a panel of changes in protein expression linked to HTLV-1 infection. Upregulation of heat shock proteins and downregulation of canonical histone expression were observed in monocytes from HTLV-1-infected patients. Moreover, expression of cytoskeleton proteins was increased in monocytes from HTLV-1-infected patients, mainly in those from HAM/TSP, which was confrmed by fow cytometry and fuorescence microscopy. Importantly, functional assays demonstrated that monocytes from HAM/TSP patients present higher ability for adhesion and transmigration thought endothelium than those from AC and uninfected individuals. The major changes on monocyte protein profle were detected in HAM/TSP patients, suggesting that these alterations exert a relevant role in the establishment of HAM/TSP. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Laboratório de Imunologia Básica e Aplicada. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro.Instituto de Bioquímica Médica Leopoldo de Meis. Unidade de Espectrometria de Massas e Proteômica. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto Nacional de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Câncer. Coordenação Geral de Ensino e Pesquisa. Programa de Oncobiologia Celular e Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Laboratório de Imunologia Básica e Aplicada. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro.Instituto de Bioquímica Médica Leopoldo de Meis. Unidade de Espectrometria de Massas e Proteômica. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro.Instituto de Bioquímica Médica Leopoldo de Meis. Unidade de Espectrometria de Massas e Proteômica. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | HTLV-1 | pt_BR |
Subject | Protein profile | pt_BR |
Subject | Blood monocytes | pt_BR |
Subject | HAM/TSP disease | pt_BR |
e-ISSN | 2045-2322 | |