Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31134
Title: Protein profile of blood monocytes is altered in HTLV-1 infected patients: implications for HAM/TSP disease
Authors: Echevarria-Lima, Juliana
Pereira, Denise de Abreu
Oliveira, Thais Silva de
Espíndola, Otávio de Melo
Lima, Marco Antonio
Leite, Ana Cláudia Celestino
Sandim, Vanessa
Nascimento, Clarissa Rodrigues
Kalume, Dario E.
Zingali, Russolina B.
Affilliation: Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Laboratório de Imunologia Básica e Aplicada. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro.Instituto de Bioquímica Médica Leopoldo de Meis. Unidade de Espectrometria de Massas e Proteômica. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto Nacional de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Câncer. Coordenação Geral de Ensino e Pesquisa. Programa de Oncobiologia Celular e Molecular. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Laboratório de Imunologia Básica e Aplicada. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro.Instituto de Bioquímica Médica Leopoldo de Meis. Unidade de Espectrometria de Massas e Proteômica. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro.Instituto de Bioquímica Médica Leopoldo de Meis. Unidade de Espectrometria de Massas e Proteômica. Rio de Janeiro, RJ, Brasil.
Abstract: Human T-cell lymphotropic virus type-1 (HTLV-1) is the etiological agent of HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). The endothelial breakdown and migration of leukocytes, including monocytes, to the spinal cord are involved in HAM/TSP development. Monocytes from HTLV-1-infected individuals exhibit important functional diferences when compared to cells from uninfected donors. Using proteomic shot gun strategy, performed by nanoACQUITY-UPLC system, we analyzed monocytes isolated from peripheral blood of asymptomatic carriers (AC), HAM/TSP and uninfected individuals. 534 proteins were identifed among which 376 were quantifed by ExpressionE software. Our study revealed a panel of changes in protein expression linked to HTLV-1 infection. Upregulation of heat shock proteins and downregulation of canonical histone expression were observed in monocytes from HTLV-1-infected patients. Moreover, expression of cytoskeleton proteins was increased in monocytes from HTLV-1-infected patients, mainly in those from HAM/TSP, which was confrmed by fow cytometry and fuorescence microscopy. Importantly, functional assays demonstrated that monocytes from HAM/TSP patients present higher ability for adhesion and transmigration thought endothelium than those from AC and uninfected individuals. The major changes on monocyte protein profle were detected in HAM/TSP patients, suggesting that these alterations exert a relevant role in the establishment of HAM/TSP.
Keywords: HTLV-1
Protein profile
Blood monocytes
HAM/TSP disease
Issue Date: 2018
Publisher: Nature Research
Citation: ECHEVARRIA-LIMA, Juliana et al. Protein profile of blood monocytes is altered in HTLV-1 infected patients: implications for HAM/TSP disease. Scientific Reports, v. 8, p. 1-14, 2018.
DOI: 10.1038/s41598-018-32324-2
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos
INI - Artigos de Periódicos

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