Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31138
Title: Monitoring fungal burden and viability of Sporothrix spp. in skin lesions of cats for predicting antifungal treatment response
Authors: Miranda, Luisa Helena Monteiro de
Silva, Jéssica Nunes
Gremião, Isabella Dib Ferreira
Menezes, Rodrigo Caldas
Almeida-Paes, Rodrigo
Reis, Érica Guerino dos
Oliveira, Raquel de Vasconcellos Carvalhaes de
Araujo, Danuza Salles do Amaral
Ferreiro, Laerte
Pereira, Sandro Antonio
Affilliation: Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Laboratório de Micologia. Porto Alegre, RS, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Epidemiologia Clínica. Fiocruz. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Laboratório de Micologia. Porto Alegre, RS, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.
Abstract: Skin lesions in feline sporotrichosis usually present a high fungal burden, making cats an important source of infection. This study evaluated the fungal burden and isolation in skin lesions of feline sporotrichosis during treatment with itraconazole (ITZ), combined with or without potassium iodide (KI). Treatment-naïve cats with culture-confirmed sporotrichosis and presenting skin ulcers were treated for up to 40 weeks with oral ITZ alone (n = 74) or combined with KI (n = 56). These cats were submitted to monthly sampling of the same lesion for mycological culture and cytopathology until healing of lesion or up to twelve weeks. The fungal burden was expressed as the mean yeast cell count in three microscopic fields from imprint smears. The fungal burden before treatment was significantly higher in cats in which the lesion persisted and in cases of treatment failure when using ITZ alone. After twelve weeks, the median fungal burden decreased to zero in both treatment protocols, suggesting a potential decrease in the risk of transmission of Sporothrix spp. from cats. These findings encourage the early treatment of feline sporotrichosis as a control measure. Moreover, the fungal burden in feline sporotrichosis lesions can be a prognostic indicator and a parameter for choosing appropriate therapeutic regimen.
Keywords: Sporotrichosis
Cat
Fungal burden
Itraconazole
Potassium iodide
Issue Date: 2018
Publisher: MDPI
Citation: MIRANDA, Luisa Helena Monteiro de et al. Monitoring fungal burden and viability of Sporothrix spp. in skin lesions of cats for predicting antifungal treatment response. Journal of Fungi, v. 4, n. 92, p. 1-11, Aug. 2018.
DOI: 10.3390/jof4030092
ISSN: 2309-608X
Copyright: open access
Appears in Collections:INI - Artigos de Periódicos

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