| Author | Fonseca, Erica L. | |
| Author | Andrade, Bruno G. N. | |
| Author | Vicente, Ana C. P. | |
| Access date | 2019-01-22T11:46:59Z | |
| Available date | 2019-01-22T11:46:59Z | |
| Document date | 2018 | |
| Citation | FONSECA, Érica L.; ANDRADE, Bruno G. N.; VICENTE, Ana C. P. The Resistome of Low-Impacted Marine Environments Is Composed by Distant Metallo-b-Lactamases Homologs. Frontiers in Microbiology, v.9, Article 677, 7p, Apr. 2018. | pt_BR |
| ISSN | 1664-302X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/31208 | |
| Language | eng | pt_BR |
| Publisher | Frontiers Media | pt_BR |
| Rights | open access | |
| Subject in Portuguese | ambiente marinho | pt_BR |
| Subject in Portuguese | gene de resistência a antibióticos | pt_BR |
| Subject in Portuguese | ambiente intocado | pt_BR |
| Title | The Resistome of Low-Impacted Marine Environments Is Composed by Distant Metallo-β-Lactamases Homologs | pt_BR |
| Type | Article | |
| DOI | 10.3389/fmicb.2018.00677 | |
| Abstract | The worldwide dispersion and sudden emergence of new antibiotic resistance genes (ARGs) determined the need in uncovering which environment participate most as their source and reservoir. ARGs closely related to those currently found in human pathogens occur in the resistome of anthropogenic impacted environments. However, the role of pristine environment as the origin and source of ARGs remains underexplored and controversy, particularly, the marine environments represented by the oceans. Here, due to the ocean nature, we hypothesized that the resistome of this pristine/low-impacted marine environment is represented by distant ARG homologs. To test this hypothesis we performed an in silico analysis on the Global Ocean Sampling (GOS) metagenomic project dataset focusing on the metallo-β-lactamases (MβLs) as the ARG model. MβLs have been a challenge to public health, since they hydrolyze the carbapenems, one of the last therapeutic choice in clinics. Using Hidden Markov Model (HMM) profiles, we were successful in identifying a high diversity of distant MβL homologs, related to the B1, B2, and B3 subclasses. The majority of them were distributed across the Atlantic, Indian, and Pacific Oceans being related to the chromosomally encoded MβL GOB present in Elizabethkingia genus. It was observed only a reduced number of metagenomic sequence homologs related to the acquired MβL enzymes (VIM, SPM-1, and AIM-1) that currently have impact in clinics. Therefore, low antibiotic impacted marine environment, as the ocean, are unlikely the source of ARGs that have been causing enormous threat to the public health. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | resistome | pt_BR |
| Subject | metallo-b-lactamases | pt_BR |
| Subject | marine environment | pt_BR |
| Subject | VIM | pt_BR |
| Subject | SPM-1 | pt_BR |
| Subject | distant homolog | pt_BR |
| Subject | antibiotic resistance gene | pt_BR |
| Subject | pristine environment | pt_BR |
| xmlui.metadata.dc.subject.ods | 14 Vida na água | |
