Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31211

Type
Article
Copyright
Open access
Collections
Share

Statistics
Metadata
Show full item record
Article has an altmetric score of 1

VALIDATION OF WESTERN BLOT FOR HISTOPLASMA CAPSULATUM ANTIBODY DETECTION ASSAY
Almeida, Marcos de Abreu et al. | Date Issued: 2016
Author
Almeida, Marcos de Abreu
Pizzini, Cláudia Vera
Damasceno, Lisandra Serra
Muniz, Mauro de Medeiros
Almeida-Paes, Rodrigo
Peralta, Regina Helena Saramago
Peralta, José Mauro
Oliveira, Raquel de Vasconcelos Carvalhaes
Vizzoni, Alexandre Gomes
Andrade, Carla Lourenço Tavares de
Zancopé-Oliveira, Rosely Maria
Affilliation
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Universidade Federal Fluminense. Departamento de Patologia. Niterói, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
Abstract
Histoplasmosis is worldwide systemic mycoses caused by the dimorphic fungus Histoplasma capsulatum. The isolation and identification of H. capsulatum in culture is the reference test for histoplasmosis diagnosis confirmation. However, in the absence of it, serology has been used as a presumptive diagnosis through antibody and antigen detection. The purpose of the present study was to validate an immunoassay method (western blot) for antibodies detection in the diagnosis of histoplasmosis.
Keywords
Histoplasmosis
Immunodiagnostic
Western blot
Sensitivity
Specificity
 
Publisher
BMC
Citation
ALMEIDA, Marcos de Abreu et al. Validation of Western Blot for histoplasma capsulatum antibody detection assay. Malaria Journal, v. 16, n. 87, p. 1-8, 2016.
DOI
10.1186/s12879-016-1427-0