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MONITORING THE PARASITE LOAD IN CHRONIC CHAGAS DISEASE PATIENTS: COMPARISON BETWEEN BLOOD CULTURE AND QUANTITATIVE REAL TIME PCR
Carga parasitária
Hemocultura
Reação em Cadeia da Polimerase em Tempo real
Pacientes
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Abstract
Despite the improvements in diagnostic tools for detection of Trypanosoma cruzi in human blood samples, the isolation of parasite from bloodstream in the chronic phase of Chagas disease is challenging. Thus, there is an increasing interest in the development of strategies that allow an accurate monitoring of the parasite load in bloodstream of Chagas disease patients. Given that, the comparison of a classical diagnostic method such as blood culture and multiplex quantitative real-time PCR (qPCR) was few explored so far. Therefore, this study aimed to compare the detection and quantification of T. cruzi load in the circulating blood of patients with chronic Chagas disease, using blood culture and qPCR techniques.
Keywords in Portuguese
Doença de ChagasCarga parasitária
Hemocultura
Reação em Cadeia da Polimerase em Tempo real
Pacientes
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