Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31307
Title: Antitumor Effect of the Essential Oil from the Leaves of Crotonmatourensis Aubl. (Euphorbiaceae)
Authors: Lima, Emilly Juliana Sales Pereira de
Alves, Rafaela G
D'Elia, Gigliola Mayara Ayres
Anunciação, Talita Andrade da
Silva, Valdenizia Rodrigues
Santos, Luciano de S
Soares, Milena Botelho Pereira
Cardozo, Nállarett Marina Dávila
Costa, Emmanoel Vilaça
Silva, Felipe M A da
Koolen, Hector Henrique Ferreira
Bezerra, Daniel Pereira
Affilliation: Amazonas State University. Metabolomics and Mass Spectrometry Research Group. Manaus, AM, Brazil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Amazonas State University. Metabolomics and Mass Spectrometry Research Group. Manaus, AM, Brazil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Center of Biotechnology and Cell Therapy. Salvador, BA, Brazil.
Amazonia Museum. Manaus, AM, Brazil.
Federal University of Amazonas. Department of Chemistry. Manaus, AM, Brazil.
Federal University of Amazonas. Department of Chemistry. Manaus, AM, Brazil.
Amazonas State University. Metabolomics and Mass Spectrometry Research Group. Manaus, AM, Brazil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Abstract: Croton matourensis Aubl. (synonym Croton lanjouwensis Jabl.), popularly known as "orelha de burro", "maravuvuia", and/or "sangrad'água", is a medicinal plant used in Brazilian folk medicine as a depurative and in the treatment of infections, fractures, and colds. In this work, we investigated the chemical composition and in vitro cytotoxic and in vivo antitumor effects of the essential oil (EO) from the leaves of C. matourensis collected from the Amazon rainforest. The EO was obtained by hydrodistillation using a Clevenger-type apparatus and characterized qualitatively and quantitatively by gas chromatography coupled to mass spectrometry (GC⁻MS) and gas chromatography with flame ionization detection (GC⁻FID), respectively. In vitro cytotoxicity of the EO was assessed in cancer cell lines (MCF-7, HCT116, HepG2, and HL-60) and the non-cancer cell line (MRC-5) using the Alamar blue assay. Furthermore, annexin V-FITC/PI staining and the cell cycle distribution were evaluated with EO-treated HepG2 cells by flow cytometry. In vivo efficacy of the EO (40 and 80 mg/kg/day) was demonstrated in C.B-17 severe combined immunodeficient (SCID) mice with HepG2 cell xenografts. The EO included β-caryophyllene, thunbergol, cembrene, p-cymene, and β-elemene as major constituents. The EO exhibited promising cytotoxicity and was able to cause phosphatidylserine externalization and DNA fragmentation without loss of the cell membrane integrity in HepG2 cells. In vivo tumor mass inhibition rates of the EO were 34.6% to 55.9%. Altogether, these data indicate the anticancer potential effect of C. matourensis.
Keywords: Croton matourensis
Croton lanjouwensis
Antitumor
Apoptosis
HepG2
keywords: Croton matourensis
Croton lanjouwensis
Antitumoral
Apoptose
HepG2
Issue Date: 2018
Publisher: MDPI
Citation: LIMA, E. J. S. P. et al. Antitumor Effect of the Essential Oil from the Leaves of Crotonmatourensis Aubl. (Euphorbiaceae). Molecules, v. 23, p. 2974, 2018.
DOI: 10.3390/molecules23112974
ISSN: 1420-3049
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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