| Author | Teixeira, John Douglas | |
| Author | Rosa, Ivone de Andrade | |
| Author | Brito, José | |
| Author | Souza, Yuli Rodrigues Maia de | |
| Author | Manso, Pedro Paulo de Abreu | |
| Author | Machado, Marcelo Pelajo | |
| Author | Costa, Manoel Luis | |
| Author | Mermelstein, Claudia | |
| Access date | 2019-01-29T15:30:16Z | |
| Available date | 2019-01-29T15:30:16Z | |
| Document date | 2018 | |
| Citation | TEIXEIRA, John Douglas; et al. Sonic Hedgehog signaling and Gli-1 during embryonic chick myogenesis. Biochemical and Biophysical Research Communications, v.507, p.496-502, Nov. 2018. | pt_BR |
| ISSN | 0006-291X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/31345 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Músculo | pt_BR |
| Subject in Portuguese | Desenvolvimento Muscular | pt_BR |
| Subject in Portuguese | Galinha | pt_BR |
| Title | Sonic Hedgehog signaling and Gli-1 during embryonic chick myogenesis | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.bbrc.2018.11.071 | |
| Abstract | The Sonic Hedgehog signaling (Shh) pathway has been implicated in both proliferation of myoblast cells
and terminal differentiation of muscle fibers, and contradictory results of these effects have been
described. To clarify the role of Shh during myogenesis, we decided to study the effects of recombinant
Shh and the distribution of Gli-1 during in vitro and in situ embryonic chick skeletal muscle differentiation
at later stages of development. Gli-1 was found in small aggregates near the nucleus in mononucleated
myoblasts and in multinucleated myotubes both in vitro and in situ chick muscle cells. Some
Gli-1 aggregates colocalized with gamma-tubulin positive-centrosomes. Gli-1 was also found in striations
and at the subsarcolemmal membrane in muscle fibers in situ. Recombinant Shh added to in vitro
grown muscle cells induced the nuclear translocation of Gli-1, as well as an increase in the number of
myoblasts and in the number of nuclei within myotubes. We suggest that Gli-1 aggregates observed in
chick muscle cells near the nuclei of myoblasts and myotubes could be a storage site for the rapid cellular
redistribution of Gli-1 upon specific signals during muscle differentiation. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Laboratório de Diferenciação Muscular e Citoesqueleto. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Laboratório de Diferenciação Muscular e Citoesqueleto. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Laboratório de Proliferação e Diferenciação Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Laboratório de Diferenciação Muscular e Citoesqueleto. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Laboratório de Diferenciação Muscular e Citoesqueleto. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Gli | pt_BR |
| Subject | Sonic Hedgehog | pt_BR |
| Subject | Muscle | pt_BR |
| Subject | Chick | pt_BR |
| Subject | Myogenesis | pt_BR |
| DeCS | Desenvolvimento Muscular | pt_BR |
| DeCS | Proteína GLI1 em Dedos de Zinco | pt_BR |
| e-ISSN | 1526-4998 | |
| Embargo date | 2030-01-01 | |
