Please use this identifier to cite or link to this item:
Type
ArticleCopyright
Open access
Collections
- IOC - Artigos de Periódicos [12734]
Metadata
Show full item record
EVALUATION OF SKIMMED MILK FLOCCULATION METHOD FOR VIRUS RECOVERY FROM TOMATOES
Author
Affilliation
Abstract
This study aimed to evaluate the elution-concentration methodology based on skimmedmilk flocculation from three varieties of tomatoes (Solanum lycopersicum L. [globe], Solanumlycopersicum var. cerasiforme [cherry] and hybrid cocktail [grape tomato]) for further moni-toring of field samples. Spiking experiments were performed to determine the success rateand efficiency recovery of human norovirus (NoV) genogroup II, norovirus murine-1 (MNV-1)used as sample process control virus and human adenovirus (HAdV). Mean values of 18.8%,2.8% and 44.0% were observed for NoV GII, MNV-1 and HAdV, respectively with differencesaccording to the types of tomatoes, with lower efficiency for cherry tomatoes. Analysis of90 samples, obtained at commercial establishments in the metropolitan region of Rio deJaneiro State, revealed 4.5% positivity for HAdV. Bacterial analysis was also performed withno detection of Salmonella spp., L. monocytogenes and fecal coliforms. Data demonstrated thatthe skimmed milk flocculation method is suitable for recovering HAdV from tomatoes andhighlights the need for considering investigation in order to improve food safety.
Share