Author | Flores, Geane Lopes | |
Author | Cruz, Helena Medina | |
Author | Miguel, Juliana Custódio | |
Author | Potsch, Denise Vigo | |
Author | Pilotto, José Henrique | |
Author | Lewis-Ximenez, Lia Laura | |
Author | Lampe, Elisabeth | |
Author | Villar, Livia Melo | |
Access date | 2019-02-05T14:05:26Z | |
Available date | 2019-02-05T14:05:26Z | |
Document date | 2018 | |
Citation | FLORES, Geane Lopes et al. Assessing hepatitisBimmunity using dried blood spot samples from HIV+ individuals. Journal of Medical Virology, v. 90, p. 1863-1867, 2018. | pt_BR |
ISSN | 0146-6615 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/31424 | |
Language | eng | pt_BR |
Publisher | Wiley | pt_BR |
Rights | restricted access | |
Subject in Portuguese | Hepatite B | pt_BR |
Subject in Portuguese | Diagnóstico | pt_BR |
Subject in Portuguese | Imunidade | pt_BR |
Subject in Portuguese | Vírus da imunodeficiência | pt_BR |
Subject in Portuguese | Mmancha de sangue seco | pt_BR |
Title | Assessing hepatitis B immunity using dried blood spot samples from HIV+ individuals | pt_BR |
Type | Article | |
DOI | 10.1002/jmv.25275 | |
Abstract | This study aims to evaluate the utility of an optimized enzyme immunoassay (EIA) to detect and quantify antibodies against hepatitis B surface antibody (anti-HBs) in dried blood spots (DBSs) within the context of human immunodeficiency virus (HIV) status. Serum and DBS samples were obtained from 56 HIV+ and 99 HIV- patients and subjected to EIA for the detection of anti-HBs, where sample volume and cut off value were modified for DBS testing. Sensitivities of anti-HBs detection in DBS were 79.8% and 76.8% in HIV- and HIV+ subjects, respectively. Concordant results for anti-HBs in serum and DBS presented high mean CD8 cell counts, HIV viral load and optical density (OD) values of anti-HBs. Anti-HBs titers were significantly higher in serum, whether or not anti-HBs titers were detected in DBS. It was possible to detect anti-HBs in DBS as low as 17.4 and 27.3 IU/mL among HIV+ and HIV- subjects, respectively. In conclusion, DBS can be used to detect and quantify anti-HBs in HIV-infected individuals, which could increase access to diagnosis and vaccination. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Ambulatório de Doenças Infecciosas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Hospital Geral de Nova Iguaçu. Departamento de Doenças Sexualmente Transmissíveis. Nova Iguaçu, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Diagnosis | pt_BR |
Subject | Dried blood spot | pt_BR |
Subject | Hepatitis B | pt_BR |
Subject | Immunodeficiency virus | pt_BR |
Subject | Immunity | pt_BR |
e-ISSN | 1096-9071 | |
Embargo date | 2030-01-01 | |