| Author | Coriolano, M. C. | |
| Author | Melo, C. M. L. de | |
| Author | Santos, A. J. G. | |
| Author | Pereira, V. R. A. | |
| Author | Coelho, L. C. B. B. | |
| Access date | 2019-02-06T12:30:35Z | |
| Available date | 2019-02-06T12:30:35Z | |
| Document date | 2012 | |
| Citation | CORIOLANO, M. C. et al. Rachycentron Canadum (Cobia) Lectin Promoted Mitogenic Response in Mice BALB/c Splenocytes. Scandinavian Journal of Immunology, v. 76, n. 6, p. 567–572, dez. 2012. | pt_BR |
| ISSN | 1365-3083 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/31447 | |
| Sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | pt_BR |
| Language | eng | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Rachycentron canadum (cobia) lectin promoted mitogenic response in mice BALB/c splenocytes | pt_BR |
| Type | Article | |
| DOI | 10.1111/j.1365-3083.2012.02774.x | |
| Abstract | The mitogenic lectins are invaluable tools to study the biochemical changes associated with lymphocyte activation and proliferation of various immune cells. Rachycentron canadum lectin (RcaL) was detected and purified from serum of cobia fish. The aim of this study was to evaluate the proliferative response and cytokine production in splenocytes of mice in vitro stimulated with RcaL lectin; Canavalia ensiformis lectin (Con A) was used as positive control. A high proliferation index was induced by RcaL in relation to control cells. Furthermore, RcaL induced higher IL-2 and IL-6 production in relation to control. The cell viability was 90% in splenocytes treated with RcaL lectin, but RcaL promoted significant late apoptosis after 24 and 48 h in relation to control. RcaL induced proliferative responses suggesting that this lectin can be used as a mitogenic agent in immunostimulatory assays. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Departamento de Bioquímica. Laboratório de Glicoproteínas da. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal Rural de Pernambuco. Departamento de Engenharia de Pesca. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Departamento de Bioquímica. Laboratório de Glicoproteínas da. Recife, PE, Brasil. | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Apoptose / efeitos de drogas | pt_BR |
| DeCS | Proliferação Celular / efeitos de drogas | pt_BR |
| DeCS | Células Cultivadas | pt_BR |
| DeCS | Imunização | pt_BR |
| DeCS | Técnicas imunológicas | pt_BR |
| DeCS | Interleucina-2 / metabolismo | pt_BR |
| DeCS | Interleucina-6 / metabolismo | pt_BR |
| DeCS | Lectinas / sangue | pt_BR |
| DeCS | Lectinas / isolamento e purificação | pt_BR |
| DeCS | Lectinas / farmacologia | pt_BR |
| DeCS | Ratos | pt_BR |
| DeCS | Ratos , endogâmicos BALB C | pt_BR |
| DeCS | Mitogênios / farmacologia | pt_BR |
| DeCS | Perciformes / sangue | pt_BR |
| DeCS | Lectinas vegetais / farmacologia | pt_BR |
| DeCS | Baço / efeitos de drogas | pt_BR |
| DeCS | Baço / imunologia | pt_BR |
| Embargo date | 2050-01-01 | |
