Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31447
Title: Rachycentron canadum (cobia) lectin promoted mitogenic response in mice BALB/c splenocytes
Authors: Coriolano, M. C.
Melo, C. M. L. de
Santos, A. J. G.
Pereira, V. R. A.
Coelho, L. C. B. B.
Affilliation: Universidade Federal de Pernambuco. Departamento de Bioquímica. Laboratório de Glicoproteínas da. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Universidade Federal Rural de Pernambuco. Departamento de Engenharia de Pesca. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Universidade Federal de Pernambuco. Departamento de Bioquímica. Laboratório de Glicoproteínas da. Recife, PE, Brasil.
Abstract: The mitogenic lectins are invaluable tools to study the biochemical changes associated with lymphocyte activation and proliferation of various immune cells. Rachycentron canadum lectin (RcaL) was detected and purified from serum of cobia fish. The aim of this study was to evaluate the proliferative response and cytokine production in splenocytes of mice in vitro stimulated with RcaL lectin; Canavalia ensiformis lectin (Con A) was used as positive control. A high proliferation index was induced by RcaL in relation to control cells. Furthermore, RcaL induced higher IL-2 and IL-6 production in relation to control. The cell viability was 90% in splenocytes treated with RcaL lectin, but RcaL promoted significant late apoptosis after 24 and 48 h in relation to control. RcaL induced proliferative responses suggesting that this lectin can be used as a mitogenic agent in immunostimulatory assays.
DeCS: Animais
Apoptose / efeitos de drogas
Proliferação Celular / efeitos de drogas
Células Cultivadas
Imunização
Técnicas imunológicas
Interleucina-2 / metabolismo
Interleucina-6 / metabolismo
Lectinas / sangue
Lectinas / isolamento e purificação
Lectinas / farmacologia
Ratos
Ratos , endogâmicos BALB C
Mitogênios / farmacologia
Perciformes / sangue
Lectinas vegetais / farmacologia
Baço / efeitos de drogas
Baço / imunologia
Issue Date: 2012
Citation: CORIOLANO, M. C. et al. Rachycentron Canadum (Cobia) Lectin Promoted Mitogenic Response in Mice BALB/c Splenocytes. Scandinavian Journal of Immunology, v. 76, n. 6, p. 567–572, dez. 2012.
DOI: 10.1111/j.1365-3083.2012.02774.x
ISSN: 1365-3083
Copyright: restricted access
Appears in Collections:PE - IAM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Coriolano_et_al-2012-Scandinavian_Journal_of_Immunology.pdf242.94 kBAdobe PDF    Request a copy



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.