Author | Moreira, Carlos José de Carvalho | |
Author | De Cicco, Nuccia Nicole Theodoro | |
Author | Galdino, Tainah Silva | |
Author | Feder, Denise | |
Author | Gonzalez, Marcelo Salabert | |
Author | Miguel, Renata Bortolasse | |
Author | Coura, José Rodrigues | |
Author | Castro, Helena Carla | |
Author | Azambuja, Patricia | |
Author | Atella, Georgia Corrêa | |
Author | Ratcliffe, Norman Arthur | |
Author | Mello, Cicero Brasileiro | |
Access date | 2019-02-07T11:45:16Z | |
Available date | 2019-02-07T11:45:16Z | |
Document date | 2018 | |
Citation | MOREIRA, Carlos José de Carvalho; et al. LipoproteinsfromvertebratehostbloodplasmaareinvolvedinTrypanosoma cruzi epimastigote agglutination and participate in interaction with the vector insect, Rhodnius prolixus. Experimental Parasitology, v.195, p.24-33, Sept. 2018. | pt_BR |
ISSN | 0014-4894 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/31468 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Subject in Portuguese | Rhodnius prolixus | pt_BR |
Subject in Portuguese | Plasma | pt_BR |
Subject in Portuguese | Aglutinação | pt_BR |
Subject in Portuguese | Plasma | pt_BR |
Subject in Portuguese | Infecção | pt_BR |
Subject in Portuguese | HDL | pt_BR |
Subject in Portuguese | Cruzipain | pt_BR |
Title | Lipoproteins from vertebrate host blood plasma are involved in Trypanosoma cruzi epimastigote agglutination and participate in interaction with the vector insect, Rhodnius prolixus | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.exppara.2018.09.017 | |
Abstract | Chagas disease, infecting ca. 8 million people in Central and South America, is mediated by the protozoan parasite, Trypanosoma cruzi. The parasite is transmitted by the bite of blood sucking triatomine insects, such as Rhodnius prolixus, that had previously fed on parasite-infected vertebrate blood and voided their contaminated feces and urine into the wound. The stages of the parasite life cycle in both the insect vector and human host are well-known, but determinants of infection in the insect gut are complex and enigmatic. This paper examines the possible role of the R. prolixus gut agglutinins in the parasite life cycle. The results, derived from gut extracts made from R. prolixus fed on various diets with different vertebrate blood components, and cross adsorption experiments, showed for the first time that R. prolixus has two distinct gut agglutinins originating from their vertebrate blood meal, one for T. cruzi (the parasite agglutinin, PA) and the other for the erythrocytes (the hemagglutinin, HA). Again, uniquely, the results also demonstrate that these two agglutinins are derived, respectively, from the plasma and erythrocyte components of the vertebrate blood. Subsequent experiments, examining in more detail the nature of the plasma components forming the T. cruzi PA, used fractionated extracts of the vertebrate plasma (high density lipoprotein, HDL; low density lipoprotein, LDL, and delipidated plasma) in agglutination assays. The results confirmed the identity of the PA as a high density lipoprotein (HDL) in the plasma of the vertebrate blood meal which agglutinates parasites in the R. prolixus gut. In addition, the use of single or double labeled HDL in fluorescence and confocal microscopy showed the interaction of the labeled HDL with the parasite surface and its internalization at later times. Finally, results of T. cruzi parasitization of R. prolixus, incorporating various vertebrate blood components, resulted in highly significant increases in infectivity in the presence of HDL from the 2nd day of infection, thus confirming the important role of this molecule in T. cruzi infection of R. prolixus. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Laboratório de Bioquímica de Lipideos e Lipoproteínas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal Fluminense. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil | pt_BR |
Affilliation | Universidade Federal Fluminense. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal Fluminense. Laboratório de Antibióticos, Bioquímica e Modelagem Molecular. Niterói, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Laboratório de Bioquímica de Lipideos e Lipoproteínas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal Fluminense. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil / Swansea University. Department of Biosciences. Swansea, Wales, UK. | pt_BR |
Affilliation | Universidade Federal Fluminense. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil. | pt_BR |
Subject | Rhodnius prolixus | pt_BR |
Subject | Trypanosoma cruzi | pt_BR |
Subject | Agglutination | pt_BR |
Subject | Plasma | pt_BR |
Subject | Cruzipain | pt_BR |
Subject | Infection | pt_BR |
Subject | HDL | pt_BR |
Embargo date | 2030-01-01 | |