Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31468
Title: Lipoproteins from vertebrate host blood plasma are involved in Trypanosoma cruzi epimastigote agglutination and participate in interaction with the vector insect, Rhodnius prolixus
Authors: Moreira, Carlos José de Carvalho
De Cicco, Nuccia Nicole Theodoro
Galdino, Tainah Silva
Feder, Denise
Gonzalez, Marcelo Salabert
Miguel, Renata Bortolasse
Coura, José Rodrigues
Castro, Helena Carla
Azambuja, Patricia
Atella, Georgia Corrêa
Ratcliffe, Norman Arthur
Mello, Cicero Brasileiro
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Laboratório de Bioquímica de Lipideos e Lipoproteínas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.
Universidade Federal Fluminense. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil
Universidade Federal Fluminense. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil.
Universidade Federal Fluminense. Laboratório de Antibióticos, Bioquímica e Modelagem Molecular. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Laboratório de Bioquímica de Lipideos e Lipoproteínas. Rio de Janeiro, RJ, Brasil.
Universidade Federal Fluminense. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil / Swansea University. Department of Biosciences. Swansea, Wales, UK.
Universidade Federal Fluminense. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil.
Abstract: Chagas disease, infecting ca. 8 million people in Central and South America, is mediated by the protozoan parasite, Trypanosoma cruzi. The parasite is transmitted by the bite of blood sucking triatomine insects, such as Rhodnius prolixus, that had previously fed on parasite-infected vertebrate blood and voided their contaminated feces and urine into the wound. The stages of the parasite life cycle in both the insect vector and human host are well-known, but determinants of infection in the insect gut are complex and enigmatic. This paper examines the possible role of the R. prolixus gut agglutinins in the parasite life cycle. The results, derived from gut extracts made from R. prolixus fed on various diets with different vertebrate blood components, and cross adsorption experiments, showed for the first time that R. prolixus has two distinct gut agglutinins originating from their vertebrate blood meal, one for T. cruzi (the parasite agglutinin, PA) and the other for the erythrocytes (the hemagglutinin, HA). Again, uniquely, the results also demonstrate that these two agglutinins are derived, respectively, from the plasma and erythrocyte components of the vertebrate blood. Subsequent experiments, examining in more detail the nature of the plasma components forming the T. cruzi PA, used fractionated extracts of the vertebrate plasma (high density lipoprotein, HDL; low density lipoprotein, LDL, and delipidated plasma) in agglutination assays. The results confirmed the identity of the PA as a high density lipoprotein (HDL) in the plasma of the vertebrate blood meal which agglutinates parasites in the R. prolixus gut. In addition, the use of single or double labeled HDL in fluorescence and confocal microscopy showed the interaction of the labeled HDL with the parasite surface and its internalization at later times. Finally, results of T. cruzi parasitization of R. prolixus, incorporating various vertebrate blood components, resulted in highly significant increases in infectivity in the presence of HDL from the 2nd day of infection, thus confirming the important role of this molecule in T. cruzi infection of R. prolixus.
Keywords: Rhodnius prolixus
Trypanosoma cruzi
Agglutination
Plasma
Cruzipain
Infection
HDL
keywords: Trypanosoma cruzi
Rhodnius prolixus
Plasma
Aglutinação
Plasma
Infecção
HDL
Cruzipain
Issue Date: 2018
Publisher: Elsevier
Citation: MOREIRA, Carlos José de Carvalho; et al. LipoproteinsfromvertebratehostbloodplasmaareinvolvedinTrypanosoma cruzi epimastigote agglutination and participate in interaction with the vector insect, Rhodnius prolixus. Experimental Parasitology, v.195, p.24-33, Sept. 2018.
DOI: 10.1016/j.exppara.2018.09.017
ISSN: 0014-4894
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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