Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31524
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dc.contributor.authorCrainey, James Lee
dc.contributor.authorMarín, Michel Abanto
dc.contributor.authorSilva, Túllio Romão Ribeiro da
dc.contributor.authorMedeiros, Jansen Fernandes de
dc.contributor.authorPessoa, Felipe Arley Costa
dc.contributor.authorSantos, Yago Vinícius
dc.contributor.authorVicente, Ana Carolina Paulo
dc.contributor.authorLuz, Sérgio Luiz Bessa
dc.date.accessioned2019-02-10T19:57:37Z
dc.date.available2019-02-10T19:57:37Z
dc.date.issued2018
dc.identifier.citationCRAINEY, James Lee et al. Mansonella ozzardi mitogenome and pseudogene characterisation provides new perspectives on filarial parasite systematics and CO-1 barcoding. Scientific Reports, v. 8, n. 6158, p.1-11, Apr. 2018.
dc.identifier.issn2045-2322
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/31524
dc.language.isoeng
dc.publisherNature Research
dc.rightsopen access
dc.subject.otherMansonella ozzardi
dc.subject.otherParasitas
dc.subject.otherPseudogenes
dc.subject.otherMetagenoma
dc.subject.otherCO-1 código de barras
dc.titleMansonella ozzardi mitogenome and pseudogene characterisation provides new perspectives on filarial parasite systematics and CO-1 barcoding
dc.typeArticle
dc.identifier.doi10.1038/s41598-018-24382-3
dc.description.abstractenDespite the broad distribution of M. ozzardi in Latin America and the Caribbean, there is still very little DNA sequence data available to study this neglected parasite's epidemiology. Mitochondrial DNA (mtDNA) sequences, especially the cytochrome oxidase (CO1) gene's barcoding region, have been targeted successfully for filarial diagnostics and for epidemiological, ecological and evolutionary studies. MtDNA-based studies can, however, be compromised by unrecognised mitochondrial pseudogenes, such as Numts. Here, we have used shot-gun Illumina-HiSeq sequencing to recover the first complete Mansonella genus mitogenome and to identify several mitochondrial-origin pseudogenes. Mitogenome phylogenetic analysis placed M. ozzardi in the Onchocercidae "ONC5" clade and suggested that Mansonella parasites are more closely related to Wuchereria and Brugia genera parasites than they are to Loa genus parasites. DNA sequence alignments, BLAST searches and conceptual translations have been used to compliment phylogenetic analysis showing that M. ozzardi from the Amazon and Caribbean regions are near-identical and that previously reported Peruvian M. ozzardi CO1 reference sequences are probably of pseudogene origin. In addition to adding a much-needed resource to the Mansonella genus's molecular tool-kit and providing evidence that some M. ozzardi CO1 sequence deposits are pseudogenes, our results suggest that all Neotropical M. ozzardi parasites are closely related.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ, Brasil / University of La Frontera. Scientific and Technological Bioresource Nucleus. Temuco, Chile.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Laboratório de Entomologia. Porto Velho, RO, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil / Programa de Pós-Graduação em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.
dc.subject.enMansonella ozzardi
dc.subject.enParasites
dc.subject.enPseudogene
dc.subject.enMetagenome
dc.subject.enCO-1 barcoding
Appears in Collections:AM - ILMD - Artigos de Periódicos
IOC - Artigos de Periódicos

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