| Author | Lourenço, Maria Cristina S. | |
| Author | Grinsztejn, Beatriz | |
| Author | Fandinho-Montes, F. C. | |
| Author | Silva, M. G. da | |
| Author | Saad, M. H. | |
| Author | Fonseca, L. S. | |
| Access date | 2019-02-11T13:43:36Z | |
| Available date | 2019-02-11T13:43:36Z | |
| Document date | 2000 | |
| Citation | LOURENÇO, Maria Cristina S. et al. Genotypic patterns of multiple isolates of M. tuberculosis from tuberculous HIV patients. Tropical Medicine and International Health, v. 5, n. 7, p. 488–494, 2000. | pt_BR |
| ISSN | 1360-2276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/31539 | |
| Language | eng | pt_BR |
| Publisher | Blackwell Science Ltd | pt_BR |
| Rights | restricted access | |
| Title | Genotypic patterns of multiple isolates of M. tuberculosis from tuberculous HIV patients | pt_BR |
| Type | Article | |
| DOI | 10.1046/j.1365-3156.2000.00583.x | |
| Abstract | We investigated whether the recurrence of tuberculosis in HIV-infected patients is due to an exogenous reinfection or relapses after antituberculosis chemotherapy. We reviewed clinical information on 32 patients at a Rio de Janeiro hospital from whom multiple Mycobacterium tuberculosis isolates were taken. All isolates were analysed by DRE-PCR fingerprinting technique, and those with identical DRE-PCR patterns were analysed by the RFLP method. Twenty patients had M. tuberculosis simultaneously isolated from different organs. These patients and nine others with sequential positive cultures after 2 months of therapy showed stable DRE-PCR and RFLP patterns. One patient's isolate became resistant to isoniazid, but the molecular pattern remained unchanged despite the development of drug resistance. In three patients, the DRE-PCR patterns of the isolates changed dramatically. Clinical and microbiological evidence was consistent with active tuberculosis caused by a new strain of M. tuberculosis. The exogenous reinfection of the three patients was not due to an outbreak, but the isolates from each patient showed unique patterns. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Bacteriologia e Bioensaios. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Tuberculosis | pt_BR |
| Subject | HIV coinfection | pt_BR |
| Subject | Multiple isolates | pt_BR |
| Subject | Genotypic patterns | pt_BR |
| Embargo date | 2030-12-31 | |
