Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31539
Title: Genotypic patterns of multiple isolates of M. tuberculosis from tuberculous HIV patients
Authors: Lourenço, Maria Cristina
Grinsztejn, Beatriz
Fandinho-Montes, F. C.
da Silva, M. G.
Saad, M. H.
Fonseca, L. S.
Affilliation: Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Bacteriologia e Bioensaios. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Desenvolvimento Tecnológico e Produtos Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.
Abstract: We investigated whether the recurrence of tuberculosis in HIV-infected patients is due to an exogenous reinfection or relapses after antituberculosis chemotherapy. We reviewed clinical information on 32 patients at a Rio de Janeiro hospital from whom multiple Mycobacterium tuberculosis isolates were taken. All isolates were analysed by DRE-PCR fingerprinting technique, and those with identical DRE-PCR patterns were analysed by the RFLP method. Twenty patients had M. tuberculosis simultaneously isolated from different organs. These patients and nine others with sequential positive cultures after 2 months of therapy showed stable DRE-PCR and RFLP patterns. One patient's isolate became resistant to isoniazid, but the molecular pattern remained unchanged despite the development of drug resistance. In three patients, the DRE-PCR patterns of the isolates changed dramatically. Clinical and microbiological evidence was consistent with active tuberculosis caused by a new strain of M. tuberculosis. The exogenous reinfection of the three patients was not due to an outbreak, but the isolates from each patient showed unique patterns.
Keywords: Tuberculosis
HIV coinfection
Multiple isolates
Genotypic patterns
Issue Date: 2000
Publisher: Blackwell Science Ltd
Citation: LOURENÇO, Maria Cristina S. et al. Genotypic patterns of multiple isolates of M. tuberculosis from tuberculous HIV patients. Tropical Medicine and International Health, v. 5, n. 7, p. 488–494, 2000.
DOI: 10.1046/j.1365-3156.2000.00583.x
ISSN: 1360-2276
Copyright: open access
Appears in Collections:INI - Artigos de Periódicos

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