Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31540
Title: Amoebiasis distribution in the past: first steps using a immunoassay technique
Authors: Gonçalves, Marcelo Luiz Carvalho
Silva, Valmir Laurentino
Andrade, Carlos Mauricio de
Reinhard, Karl Jan
Rocha, Gino Chaves da
Bailly, Matthieu Le
Bouchet, Françoise
Silva, Luiz Fernando Rocha Ferreira da
Araújo, Adauto José Goncalves de
Affilliation: Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
University of Nebraska. School of Natural Resource Sciences. Lincoln, NE, USA.
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil / Universite de Reims. U. F. R. de Pharmacie. Laboratoire de Paleoparasitologie. Reims, France
Universite de Reims. U. F. R. de Pharmacie. Laboratoire de Paleoparasitologie. Reims, France.
Universite de Reims. U. F. R. de Pharmacie. Laboratoire de Paleoparasitologie. Reims, France.
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
Abstract: The identification of parasites in ancient human faeces is compromised by differential preservation of identifiable parasite structures. However, protein molecules can survive the damage of the environment and can be detected even after centuries. In this paper it is shown that is possible to detect copro-antigen of Entamoeba histolytica in historic and prehistoric human faecal remains, using a commercially available enzyme immunoassay (ELISA) kit. The kit uses monoclonal antibody-peroxidase conjugate specific for E. histolytica adhesin. A total of 90 specimens of desiccated faeces found in mummies and ancient organic sediment from South America, North America, Africa, and Europe were examined. The ELISA detected 20 positive samples, dated to about 5300 years before present to the 19th Century ad. The positive samples are from archaeological sites in Argentina, USA, France, Belgium, and Switzerland. The detection of protozoan antigen using immunoassays is a reliable tool for the studies of intestinal parasites in the past.
Keywords: Paleoparasitology
Coprolites
Ancient Faeces
Amebiasis
ELISA
DeCS: Amebiasis
Enzyme-Linked Immunosorbent Assay
Issue Date: 2004
Publisher: Transactions of The Royal Society of Tropical Medicine and Hygiene
Citation: GONÇALVES, Marcelo Luiz Carvalho et al. Amoebiasis distribution in the past: first steps using an immunoassay technique. Transactions of The Royal Society of Tropical Medicine and Hygiene, v. 98, n. 2, p. 1-4, 2004
ISSN: 0035-9203
Copyright: open access
Appears in Collections:ENSP - Artigos de Periódicos

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