Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31614
Title: Occurrence of uterine carriers for Leptospira interrogans on slaughtered cows
Authors: Pires, Bruno Cabral
Grapiglia, Junia Berzin
Moreira, Lio
Jaeger, Lauren Hubert
Costa, Filipe Anibal Carvalho
Lilenbaum, Walter
Affilliation: Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Bacteriologia Veterinária. Niterói, Rj, Brasil.
Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Bacteriologia Veterinária. Niterói, Rj, Brasil.
Universidade Federal do Estado do Rio de Janeiro. Departamento de Microbiologia e Parasitologia. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.
Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Bacteriologia Veterinária. Niterói, Rj, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil.
Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Bacteriologia Veterinária. Niterói, Rj, Brasil.
Abstract: Reproductive tract is an important site of infection for chronic leptospirosis and cooperate in pathogenesis of reproductive failure, leading to economic losses. Since serology techniques cannot detect chronic carriers, the molecular analysis of clinical samples is an alternative to detect these animals on livestock. The aim of the present study was to perform a retrospective study in order to detect leptospiral uterine carriers in slaughtered cows. Tissue samples were collected from 50 post-pubertal, nonpregnant cows. These samples were fixed in 10% buffered formalin, paraffin-embedded and stored. PCR targeting lipL32 gene and molecular characterization by secY sequence was performed. Leptospiral DNA was identified in 18% (9/50) examined blocks. Two sequences were characterized as L. interrogans. These findings suggest that the presence of infectious leptospires in uterus is associated with the physiopathogenesis of the reproductive failure.
Keywords: Leptospirosis
Genital tract
Uterus
SecY
Sequencing
keywords: Leptospirose
Útero
Sequência
Trato genital
Issue Date: 2018
Publisher: Elsevier
Citation: PIRES, Bruno Cabral; et al. Occurrence of uterine carriers for Leptospira interrogans on slaughtered cows. Microbial Pathogenesis, v.114, p.163-165, Nov. 2018.
DOI: 10.1016/j.micpath.2017.11.056
ISSN: 0882-4010
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Filpeanibal_costa_etal_IOC_2018.pdf230.53 kBAdobe PDF    Request a copy


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.