Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31646
Title: Diagnosis of disseminated mycobacterial infection: testing a simple and inexpensive method for use in developing countries
Other Titles: Diagnostic de la mycobacteriose generalisee: essai d'une methode simple et bon marche utilisable dans les pays en developpement
Authors: Fandinho, Fátima C.
Grinsztejn, Beatriz
Veloso, Valdiléa G.
Lourenço, Maria Cristina
Werneck-Barroso, Eduardo
João, Esaú
Nogueira, S. A.
Fonseca, L. de S.
Affilliation: Fundação Oswaldo Cruz. Instituto de Produtos Imunobiológicos. Departamento de Desenvolvimento Tecnológico./ Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Bacteriologia e Bioensaios. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas./ Universidade Federal do Rio de Janeiro. Instituto de Pneumologia e Fisiologia. Rio de Janeiro, RJ, Brasil.
Hospital dos Servidores do Estado do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Departamento de Doenças Infecciosas. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Microbiologia Médica. Rio de Janeiro, RJ, Brasil.
Abstract: With the development of the acquired immunodeficiency syndrome (AIDS) epidemic, the isolation of mycobacteria from blood has become a common problem for clinical laboratories. In this study two methods were used for the recovery of mycobacteria from blood specimens obtained from AIDS patients: (1) direct inoculation of a biphasic medium, and (2) a non-commercial lysis-centrifugation method. A total of 3 consecutive blood samples were taken at 15-minute intervals from each of 50 AIDS patients with clinical suspicion of disseminated mycobacterial disease. Mycobacterium growth was noted in 70/138 blood specimens from 30 (60%) patients. These cultures yielded Mycobacterium tuberculosis in 19 (63%) and Mycobacterium avium complex organisms in 11 (37%) patients. Cultures using the lysis-centrifugation method were positive in 54% of the patients while cultures using biphasic medium were positive in 44% (P > 0.05). The positivity for M. avium complex was higher with lysis-centrifugation (91%) than with biphasic medium (45.4%) (P < 0.05). However, the positivities for M. tuberculosis with the lysis-centrifugation method (89.5%) and direct inoculation in biphasic medium (100%) were similar (P > 0.05). The use of a non-commercial lysis-centrifugation technique is inexpensive, reliable, and can be an alternative method for the diagnosis of mycobacteraemia in developing countries.
???metadata.dc.description.abstractfr???: Avec le developpement de l'6pid6mie de syndrome d'immunod6ficience acquise (SIDA), l'isolement des mycobact6ries dans le sang est devenu un probleme fr6quent pour les laboratoires d'analyses m6dicales. Deux methodes ont et6 utilisees dans la presente 6tude pour cultiver les mycobact6ries a partir des pr6levements de sang pratiqu6s chez les patients atteints de SIDA: 1) inoculation directe d'un milieu biphasique, et 2) m6thode non commercialis6e par lyse et centrifugation. Au total, trois 6chantillons de sang consecutifs ont ete pr6leves a 15 minutes d'intervalle chez chacun des 50 patients pr6sum6s cliniquement atteints de mycobact6riose gen6ralisee. Les mycobacteries ont cultive pour 70 des 138 prelevements de sang realis6s chez 30 patients (soit 60%). Ces cultures ont permis d'obtenir Mycobacterium tuberculosis chez 19 patients (soit 63%), et des germes appartenant au complexe Mycobacterium avium chez 1 1 patients (soit 37%). Les cultures obtenues par lyse-centrifugation etaient positives chez 54% des patients, tandis que les cultures sur milieu biphasique 6taient positives chez 44% des patients (p > 0,05). Le nombre de cultures positives pour le complexe M. avium 6tait plus grand avec la lyse-centrifugation (91%) qu'avec le milieu biphasique (45,4%) (p < 0,05). Pour M. tuberculosis, la sensibilit6 de la lysecentrifugation (89,5%) et celle de l'inoculation directe en milieu biphasique (100%) 6taient toutefois comparables (p > 0,05). La lyse-centrifugation non commercialisee est une technique bon marche, fiable, et qui peut etre utilis6e comme m6thode de remplacement pour le diagnostic de la mycobact6ri6mie dans les pays en developpement.
Keywords: AIDS
Mycobacterium avium
Mycobacterium tuberculosis
Issue Date: 1997
Publisher: World Health Organization
Citation: FANDINHO, F. C. et al. Diagnosis of disseminated mycobacterial infection: testing a simple and inexpensive method for use in developing countries. Bulletin of the World Health Organization, v. 75, n. 4, p. 361-366, 1997.
ISSN: 0042-9686
Copyright: open access
Appears in Collections:INI - Artigos de Periódicos

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