Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31683
Title: Co-infection with feline retrovirus is related to changes in immunological parameters of cats with sporotrichosis
Authors: Miranda, Luisa Helena Monteiro de
Meli, Marina
Silva, Fátima Conceição
Novacco, Marilisa
Menezes, Rodrigo Caldas
Pereira, Sandro Antonio
Sugiarto, Sarah
Reis, Érica Guerino dos
Gremião, Isabella Dib Ferreira
Hofmann-Lehmann, Regina
Affilliation: Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil / University of Zurich. Vetsuisse Faculty. Clinical Laboratory and Center for Clinical Studies. Zurich, Switzerland.
University of Zurich. Vetsuisse Faculty. Clinical Laboratory and Center for Clinical Studies. Zurich, Switzerland.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.
University of Zurich. Vetsuisse Faculty. Clinical Laboratory and Center for Clinical Studies. Zurich, Switzerland.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
University of Zurich. Vetsuisse Faculty. Clinical Laboratory and Center for Clinical Studies. Zurich, Switzerland.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
University of Zurich. Vetsuisse Faculty. Clinical Laboratory and Center for Clinical Studies. Zurich, Switzerland.
Abstract: Feline sporotrichosis due to Sporothrix brasiliensis is frequently severe and often correlated to zoonotic transmission. Feline Immunodeficiency Virus (FIV) and Feline Leukemia Virus (FeLV) cause immunodeficiency in cats; no association has been identified with critical cases of sporotrichosis. Moreover, the cytokine profile in Sporothrix-infected cats and a potential impact of retrovirus co-infections on their immunity is unknown. This study assessed immunological parameters in cats with sporotrichosis with and without FIV or FeLV co-infection. FeLV infection was detected by antigen ELISA and by provirus PCR. FIV infection was investigated through ELISA and Western blot. Cytokine transcription (IFN-γ, IL-4, IL-5, IL-6, IL-10, IL-12, TNF-α) was quantified using RT-qPCR and lymphocyte subpopulations (CD4, CD8, CD5 and CD21) were assessed by flow cytometry. Thirty cats with sporotrichosis were recruited to the study, including three FIV-positive and five FeLV-positive (progressive infection) cats. One cat with regressive FeLV infection was excluded from statistics. In comparison to retrovirus-negative cats, FIV-positive cats and FeLV-positive cats had higher IL-10 levels, FeLV-positive cats had lower IL-4 levels and FIV-positive cats had lower IL-12 levels and a lower CD4+/CD8+ ratio. Remarkably, all cats with poor general condition were FeLV (progressive infection) or FIV-positive, but the retrovirus status was not associated with the sporotrichosis treatment length or outcome. The immunological changes and the more severe clinical presentation observed in cats with retrovirus co-infections encourage future prospective studies that address the impact of these changes on prognostic determinants of feline sporotrichosis and the development of new therapy strategies that control disease spread.
Keywords: Co-infection
Feline retrovirus
Immunological parameters
Sporotrichosis
Cats
keywords: Esporotricose
Co-infecção
Parâmetros imunológicos
Gatos
Retrovírus felino
Issue Date: 2018
Publisher: Public Library of Science
Citation: MIRANDA, Luisa Helena Monteiro de et al. Co-infection with feline retrovirus is related to changes in immunological parameters of cats with sporotrichosis. Plos One, v. 13, n. 11, p. 1-16, Nov. 2018.
DOI: 10.1371/journal.pone.0207644
ISSN: 1932-6203
Copyright: open access
Appears in Collections:INI - Artigos de Periódicos
IOC - Artigos de Periódicos

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