Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31977
Title: Lack of chitin synthase genes impacts capsular architecture and cellular physiology in Cryptococcus neoformans
Authors: Rodrigues, Jessica
Ramos, Caroline L.
Frases, Susana
Godinho, Rodrigo M. da C.
Fonseca, Fernanda L.
Rodrigues, Marcio L.
Affilliation: Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brazil.
Abstract: Cryptococcus neoformans mutants lacking each of the eight putative chitin synthase genes (CHS) have been previously generated. However, it is still unclear how deletion of chitin synthase genes affects the cryptococcal capsule. Since the connections between chitin metabolism and capsular polysaccharides in C. neoformans are numerous, we analyzed the effects of deletion of CHS genes on capsular and capsule-related structures of C. neoformans. CHS deletion affected capsular morphology in multiple ways, as determined by scanning electron microscopy and immunofluorescence analysis. Molecular diameter, serological reactivity and export of capsular polysaccharide were also affected in most of the chsΔ mutants, but the most prominent alterations were observed in the chs3Δ strain. C. neoformans cells lacking CHS genes also had altered formation of extracellular vesicles and variable chitinase activity under stress conditions. These results reveal previously unknown functions of CHS genes that greatly impact the physiology of C. neoformans.
Keywords: Cryptococcus neoformans
Chitin synthase genes (CHS)
Cellular physiology
Issue Date: 2018
Publisher: Elsevier
Citation: RODRIGUES, J. et al. Lack of chitin synthase genes impacts capsular architecture and cellular physiology in Cryptococcus neoformans. The Cell Surface, v. 2, p. 14-23, 2018.
DOI: 10.1016/j.tcsw.2018.05.002
ISSN: 2468-2330
Copyright: restricted access
Appears in Collections:PR - ICC - Artigos de Periódicos
CDTS - Artigos de Periódicos

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