Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32003
Title: Detection and clearance of a mosquito densovirus contaminant from laboratory stocks of Zika virus
Authors: Cataneo, Allan Henrique Depieri
Kuczera, Diogo
Mosimann, Ana Luiza Pamplona
Silva, Emanuele Guimarães
Ferreira, Álvaro Gil Araújo
Marques, João Trindade
Wowk, Pryscilla Fanini
Santos, Claudia Nunes Duarte Dos
Bordignon, Juliano
Affilliation: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Abstract: The Zika virus (ZIKV) epidemics that affected South America in 2016 raised several research questions and prompted an increase in studies in the field. The transient and low viraemia observed in the course of ZIKV infection is a challenge for viral isolation from patient serum, which leads to many laboratories around the world sharing viral strains for their studies. C6/36 cells derived from Aedes albopictus larvae are commonly used for arbovirus isolation from clinical samples and for the preparation of viral stocks. Here, we report the contamination of two widely used ZIKV strains by Brevidensovirus, here designated as mosquito densovirus (MDV). With methodology, Molecular and immunological techniques were used to analyse the MDV contamination of ZIKV stocks. Also, virus passages in mammalian cell line and infecting susceptible mice were used to MDV clearance from ZIKV stocks. MDV contamination was confirmed by molecular and immunological techniques and likely originated from C6/36 cultures commonly used to grow viral stocks. We applied two protocols that successfully eliminated MDV contamination from ZIKV stocks, and these protocols can be widely applied in the field. As MDV does not infect vertebrate cells, we performed serial passages of contaminated stocks using a mammalian cell line and infecting susceptible mice prior to re-isolating ZIKV from the animals’ blood serum. MDV elimination was confirmed with immunostaining, polymerase chain reaction (PCR), and analysis of the mosquitoes that were allowed to feed on the infected mice. How main conclusion since the putative impact of viral contaminants in ZIKV strains generally used for research purposes is unknown, researchers working in the field must be aware of potential contaminants and test viral stocks to certify sample purity.
Keywords: Arboviruses
Contamination
Zika Virus
Keywords in spanish: Arbovirus
Contaminación
Virus Zika
keywords: Densovirus
DeCS: Arbovirus
Densovirus
Contaminação
Issue Date: 2019
Publisher: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
Citation: CATANEO, Allan Henrique Depieri et al. Detection and clearance of a mosquito densovirus contaminant from laboratory stocks of Zika virus. Mem. Inst. Oswaldo Cruz, v. 114, p. 1-8, 2019.
DOI: 10.1590/0074-02760180432
ISSN: 1678-8060
Copyright: open access
Appears in Collections:PR - ICC - Artigos de Periódicos

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