Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32007
Title: Presence of leptospires on genital tract of mares with reproductive problems
Authors: Hamond, Camila
Pestana, Cristiane P.
Souza, Cláudio Marcos Rocha de
Cunha, Luis Eduardo R.
Brandão, Felipe Z.
Medeiros, Marco Alberto
Lilenbaum, Walter
Affilliation: Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Bacteriologia Veterinária. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Instituto Vital Brasil. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Faculdade de Medicina Veterinária. Departamento de Patologia e Veterinária Clínica, Faculdade de Veterinária. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Universidade Federal Fluminense.Departamento de Microbiologia e Parasitologia. Laboratório de Bacteriologia Veterinária. Niterói, RJ, Brasil.
Abstract: Leptospirosis is a zoonotic disease of global importance, and has a worldwide distribution. Equine leptospirosis is commonly manifested by recurrent uveitis, reproductive disorders, as abortions, embryonic absorption, stillbirth and the birth of weak foals. The aim of this study was to verify the presence of Leptospira sp or its DNA in genital tract of mares with reproductive problems. A total of 38 mares with reproductive problems were studied. All the mares were sampled for blood (for serology), urine (for culturing and qPCR), vaginal fluid-VF and endometrial biopsy-EB (for culturing, qPCR and indirect immunofluorescence). PCRs products were sequenced for secY gene. Seventeen (44.7%) serum samples were reactive, predominantly against serogroups Australis (76.4%) and Pomona (23.6%). No positive culture was obtained, but DNA was detected by qPCR on urine samples (26.3%), VF (44.7%) and EB (18.4%) collected 2 months or longer following diagnosis of early fetal death and endometritis. Leptospira cell aggregations were visible by indirect immunofluorescence on 57.1% (4/7) EBs and 17.6% (3/17) VFs. A total of 18 amplicons showed interpretable sequences. Out of those 18 amplicons, 15 presented 100% of identity with the species L. interrogans (sv Bratislava and Pomona), while three were L. borgpertersenii. This study suggests the presence of leptospires in the uterus of mares with reproductive problems. Moreover, serology was shown not to be indicated for the diagnosis of presumptive Leptospira infection in early gestation. The most common agent of the genital infection in those mares was L. interrogans, most probably sg Australis.
Keywords: Horse
Leptospira
Uterus
Issue Date: 2015
Citation: HAMOND, Camila et al. Presence of leptospires on genital tract of mares with reproductive problems. Veterinary Microbiology, Amsterdam, v. 179, n. 3-4, p. 264-269, 2015.
Description: Artigo em acesso aberto no link da Editora.
DOI: 10.1016/j.vetmic.2015.06.014
ISSN: 1873-2542
Copyright: open access
Appears in Collections:Biomanguinhos - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
1-s2.0-S0378113515002503-main.pdf600.3 kBAdobe PDFView/Open


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.