| Author | Leles, Daniela | |
| Author | Araújo, Adauto | |
| Author | Ferreira, Luiz Fernando | |
| Author | Vicente, Ana Carolina Paulo | |
| Author | Iñiguez, Alena Mayo | |
| Access date | 2019-03-09T18:52:30Z | |
| Available date | 2019-03-09T18:52:30Z | |
| Document date | 2008 | |
| Citation | LELES, Daniela et al. Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Columbian South America times. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 1, p. 106-108, Feb. 2008. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/32020 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Ascaris | pt_BR |
| Subject in Portuguese | Paleodistribuição | pt_BR |
| Subject in Portuguese | Diagnóstico paleoparasitológico molecular | pt_BR |
| Title | Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Columbian South America times | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0074-02762008005000004 | pt_BR |
| Abstract | Paleoparasitological studies using microscopy showed that Ascaris and Trichuris trichiura are the human intestinal parasites most found in archaeological sites. However, in pre-Columbian South American archaeological sites, Ascaris is rare. In this work we standardized a molecular methodology for Ascaris diagnosis directly from ancient DNA retrieved from coprolites. Using cythochrome b gene (142 bp) target, ancient DNA sequences were retrieved from South American samples, negative by microscopy. Moreover, the methodology applied was sensitive enough to detect ancient DNA extracted from 30 Ascaris eggs from an European coprolite. These results revealed a new scenery for the paleodistribution of Ascaris in South America. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro. RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro. RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro. RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Ascaris | pt_BR |
| Subject | Molecular paleoparasitological diagnosis | pt_BR |
| Subject | Paleodistribution | pt_BR |
| e-ISSN | 1678-8060 | |
