Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32020
Title: Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Columbian South America times
Authors: Leles, Daniela
Araújo, Adauto
Ferreira, Luiz Fernando
Vicente, Ana Carolina Paulo
Iñiguez, Alena Mayo
Affilliation: Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro. RJ, Brasil
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro. RJ, Brasil
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro. RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Abstract: Paleoparasitological studies using microscopy showed that Ascaris and Trichuris trichiura are the human intestinal parasites most found in archaeological sites. However, in pre-Columbian South American archaeological sites, Ascaris is rare. In this work we standardized a molecular methodology for Ascaris diagnosis directly from ancient DNA retrieved from coprolites. Using cythochrome b gene (142 bp) target, ancient DNA sequences were retrieved from South American samples, negative by microscopy. Moreover, the methodology applied was sensitive enough to detect ancient DNA extracted from 30 Ascaris eggs from an European coprolite. These results revealed a new scenery for the paleodistribution of Ascaris in South America.
Keywords: Ascaris
Molecular paleoparasitological diagnosis
Paleodistribution
keywords: Ascaris
Paleodistribuição
Diagnóstico paleoparasitológico molecular
Issue Date: 2008
Publisher: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
Citation: LELES, Daniela et al. Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Columbian South America times. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 1, p. 106-108, Feb. 2008.
DOI: 10.1590/S0074-02762008005000004
ISSN: 0074-0276
Copyright: open access
Appears in Collections:ENSP - Artigos de Periódicos
IOC - Artigos de Periódicos

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