Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32038
Title: Development of a membrane adsorber based capture step for the purification of yellow fever virus
Authors: Pato, Tânia P.
Souza, Marta Cristina O.
Silva, Andréa N. M. R
Pereira, Renata C.
Silva, Marlon V.
Caride, Elena
Gaspar, Luciane P.
Freire, Marcos S.
Castilho, Leda R.
Affilliation: Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Programa de Bioquímica. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. COPPE. Laboratório de Engenharia de Cultura Celular. Rio de Janeiro , RJ, Brasil / Universidade Federal do Rio de Janeiro. Programa de Bioquímica. Rio de Janeiro, RJ, Brasil.
Abstract: Yellow fever (YF) is an endemic disease in some tropical areas of South America and Africa that presents lethality rate between 20 and 50%. There is no specific treatment and to control this disease a highly effective live-attenuated egg based vaccine is widely used for travelers and residents of areas where YF is endemic. However, recent reports of rare, sometimes fatal, adverse events post-vaccination have raised concerns. In order to increase safety records, alternative strategies should be considered, such as developing a new inactivated vaccine using a cell culture based technology, capable of meeting the demands in cases of epidemic. With this goal, the production of YF virus in Vero cells grown on microcarriers and its subsequent purification by chromatographic techniques was studied. In this work we investigate the capture step of the purification process of the YF virus. At first, virus stability was studied over a wide pH range, showing best results for the alkaline region. Considering this result and the pI of the envelope protein previously determined in silico, a strong anion exchanger was considered most suitable. Due to the easy scalability, simplicity to handle, absence of diffusional limitations and suitability for virus handling of membrane adsorbers, a Q membrane was evaluated. The amount of antigen adsorbed onto the membrane was investigated within the pH range for virus stability, and the best pH for virus adsorption was considered to be 8.5. Finally, studies on gradient and step elution allowed to determine the most adequate salt concentration for washing (0.15 M) and virus elution (0.30 M). Under these operating conditions, it was shown that this capture step is quite efficient, showing high product recovery (93.2 ± 30.3%) and efficient DNA clearance (0.9 ± 0.3 ng/dose).
Keywords: Yellow fever virus
Yellow fever
Endemic disease
Epidemic
Issue Date: 2014
Citation: PATO, Tânia P. et al. Development of a membrane adsorber based capture step for the purification of yellow fever virus. Vaccine, Amsterdam, v. 32, n. 24, p. 2789-2793, 2014.
DOI: 10.1016/j.vaccine.2014.02.036
ISSN: 1873-2518
Copyright: open access
Appears in Collections:Biomanguinhos - Artigos de Periódicos

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