Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32084
Title: Histology and Ultrastructure of Aedes albopictus Larval Midgut Infected with Bacillus thuringiensis var. israelensis
Authors: Silva, Vanderlei C.
Pinheiro, Nadja L.
Scherer, Paulo O.
Falcão, Simone S.
Ribeiro, Vinicius R.
Mendes, Rosa Maria M.
Chagas, Rodrigo
Almeida, Margareth Cardozo de
Santos-Mallet, Jacenir R.
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência para Vigilância Entomológica: Taxonomia e Ecologia de Vetores de Leishmaniose. Rio de Janeiro, RJ. Brasil.
Universidade Federal Rural do Rio de Janeiro. Instituto de Biologia. Programa de Pós-Graduação em Biologia Animal. Seropédica, RJ, Brasil.
Universidade Federal Rural do Rio de Janeiro. Instituto de Biologia. Programa de Pós-Graduação em Biologia Animal. Seropédica, RJ, Brasil.
Universidade Federal Rural do Rio de Janeiro. Instituto de Biologia. Programa de Pós-Graduação em Biologia Animal. Seropédica, RJ, Brasil.
Universidade Federal Rural do Rio de Janeiro. Instituto de Biologia. Programa de Pós-Graduação em Biologia Animal. Seropédica, RJ, Brasil.
Universidade Federal Rural do Rio de Janeiro. Instituto de Biologia. Programa de Pós-Graduação em Biologia Animal. Seropédica, RJ, Brasil.
Universidade Federal Rural do Rio de Janeiro. Instituto de Biologia. Programa de Pós-Graduação em Biologia Animal. Seropédica, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência para Vigilância Entomológica: Taxonomia e Ecologia de Vetores de Leishmaniose. Rio de Janeiro, RJ. Brasil / Universidade Federal Rural do Rio de Janeiro. Instituto de Biologia. Programa de Pós-Graduação em Biologia Animal. Seropédica, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência para Vigilância Entomológica: Taxonomia e Ecologia de Vetores de Leishmaniose. Rio de Janeiro, RJ. Brasil.
Abstract: Histological and ultrastrucutural alterations in the midgut of Aedes albopictus larvae infected with Bacillus thuringiensis var. israelensis (Bti) were observed by light and transmission electron microscopy (TEM). Two formulations of Bti were used: granulated and powder, with 0.2% active ingredient in 90 larvae of Ae. albopictus distributed in three containers containing 30 larvae each (one control group and two test groups). The midgut epithelium of the control group presented flattened and elongated cells with mace-shape with a narrow base. Midgut epithelium cells’ surface was convex and had a large circular nucleus located in the median-apical portion of the cell. These cells also presented a basal lamina with a small accumulation of extracellular fibrous matrix, thus characterizing a basal membrane, with a muscle layer and a peritoneal membrane more externally. After Bti ingestion, the larvae stopped/slowed their natural movements down in 5 min. After 30 min approximately, the swimming movements stopped completely. Internally, the intestinal cells showed a disorganization of the basal processes, dilatation and fragmentation of the rough endoplasmic reticulum, with intense cytoplasmic vacuolization. There were concentric dense laminas accumulated in the cytoplasm, and these residual membranous bodies were seen greatly increased in size after 60 min. Mitochondria, fragments of rough endoplasmic reticulum and other remainder organelles were surrounded and segregated from the cytoplasm by exocytosis. This article reports the histopathological alterations in the midgut of Ae. albopictus after infection with Bti and contributes to a better understanding of the mode of action of this bacterial strain used as bioinsecticide against mosquito larvae.
Keywords: Diptera
Biological control
midgut
Culicidae
keywords: Diptera
Culicidae
Controle biológico
Intestino
Issue Date: 2008
Publisher: Wiley 12 Months
Citation: SILVA, Vanderlei C. et al. Histology and Ultrastructure of Aedes albopictus Larval Midgut Infected with Bacillus thuringiensis var. israelensis. Microscopy Research and Technique, v.71, p.663-668, 2008.
DOI: 10.1002/jemt.20605
ISSN: 1059-910X
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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