Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32093
Title: Pattern of metalloprotease activity and myofiber regeneration in skeletal muscles of mdx mice
Authors: Bani, Cristiane
Lagrota-Candido, Jussara
Pinheiro, Douglas Florindo
Leite, Paulo Emílio Correa
Salimena, Maria Cristina
Henriques-Pons, Andrea
Quirico-Santos, Thereza
Affilliation: Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. Departamento Imunobiologia. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. Departamento Imunobiologia. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura. Rio de Janeiro, RJ. Brasil.
niversidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.
Abstract: Matrix metalloproteases (MMPs) are key regulatory molecules in the formation, remodeling, and degradation of extracellular matrix components in both physiological and pathological processes. Skeletal muscles of mdx dystrophic mice show distinct patterns of inflammation and regeneration, suggesting that factors within the microenvironment influence the adaptive responses of muscles with predominantly slow-twitch or fast-twitch fibers. This study aimed to verify the pattern of MMP activity in gastrocnemius, soleus, and diaphragm muscles and correlate it with the regenerative capability at distinct stages of the mdx myopathy. Marked inflammation and myonecrosis was associated with increased MMP-9 activity and TNF-alpha (tumor necrosis factor-alpha) production, whereas muscle regeneration, evidenced by NCAM (neural cell adhesion molecule) expression and MMP-2 activity, varied at different stages of the disease. Soleus muscles showed a high percentage of NCAM-positive myofibers in the early stages (2 weeks) of the disease, but they appeared in the gastrocnemius muscles at 12 weeks and in the diaphragm at 24 weeks. Increased MMP-2 activity in the diaphragm throughout all stages of the disease suggests important tissue remodeling, which is probably associated with persistent inflammation. The results indicate that the microenvironment of distinct skeletal muscle may influence a particular kinetic pattern of MMP activity, which ultimately favors persistent inflammation and myofiber regeneration at different stages of the myopathy in mdx mice.
Keywords: Metalloprotease
Muscle regeneration
Muscular dystrophy
Myofiber type
keywords: Metaloprotease
Regeneração muscular
Distrofia muscular
Tipo de miofibra
Issue Date: 2008
Publisher: Wiley 12 Months
Citation: BANI, Cristiane et al. Pattern of metalloprotease activity and myofiber regeneration in skeletal muscles of mdx Mice. Muscle Nerve, v. 37, p. 583-592, 2008.
DOI: 10.1002/mus.20970
ISSN: 0148-639X
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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