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MIXED INFECTION BY HISTOPLASMA CAPSULATUM ISOLATES WITH DIFFERENT MATING TYPES IN BRAZILIAN AIDS-PATIENTS
Damasceno, Lisandra Serra et al. | Date Issued: 2019
Author
Damasceno, Lisandra Serra
Vite-Garín, Tania
Ramírez, José Antonio
Rodríguez-Arellanes, Gabriela
Almeida, Marcos Abreu de
Muniz, Mauro de Medeiros
Mesquita, Jacó Ricarte Lima de
Leitão, Terezinha do Menino Jesus Silva
Taylor, Maria Lucia
Zancopé-Oliveira, Rosely Maria
Affilliation
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Setor Imunodiagnóstico. Rio de Janeiro, RJ, Brasil / Hospital São José de Doenças Infecciosas. Fortaleza, CE, Brasil.
Universidad Nacional Autónoma de México. Facultad de Medicina. Departamento de Microbiología y Parasitología. Laboratorio de Inmunología de Hongos. Ciudad de México, México
Universidad Nacional Autónoma de México. Facultad de Medicina. Departamento de Microbiología y Parasitología. Laboratorio de Inmunología de Hongos. Ciudad de México, México
Universidad Nacional Autónoma de México. Facultad de Medicina. Departamento de Microbiología y Parasitología. Laboratorio de Inmunología de Hongos. Ciudad de México, México
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Setor Imunodiagnóstico. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Setor Imunodiagnóstico. Rio de Janeiro, RJ, Brasil.
Hospital São José de Doenças Infecciosas. Fortaleza, CE, Brasil.
Hospital São José de Doenças Infecciosas. Fortaleza, CE, Brasil / Universidade Federal do Ceará. Faculdade de Medicina. Departamento de Saúde Comunitária. Fortaleza, CE, Brasil.
Universidad Nacional Autónoma de México. Facultad de Medicina. Departamento de Microbiología y Parasitología. Laboratorio de Inmunología de Hongos. Ciudad de México, México
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Setor Imunodiagnóstico. Rio de Janeiro, RJ, Brasil.
Abstract
Mixed infection by Histoplasma capsulatum isolates with different mating types, in AIDS‑patients are described in this study. Morphological, mating type-specific PCR assay and multilocus sequencing type analysis of H. capsulatum isolates recovered from two Brazilian AIDS‑patients were performed. Five H. capsulatum isolates were recovered at different times from the two patients. Three isolates were obtained from bone marrow (day 1 – CE0411) and buffy coat cultures (day 1 – CE0311; day 2 – CE0511) of patient 1, and two isolates were isolated from buffy coat cultures (day 3 – CE2813; day 12 – CE2513) of patient 2. The mycelial colonies depicted different textures and pigmentation features. Dimorphic conversion to the yeast-phase in ML-Gema medium was achieved in all isolates. MAT1-1 idiomorph was identified in CE0311, CE0411 and CE2813 isolates; MAT1-2 idiomorph was found in CE0511 and CE2513 isolates. These H. capsulatum isolates were grouped within LAm A clade, highlighting that CE0311 and CE0411 isolates formed a subgroup supported by a high bootstrap value. The CE0511, CE2513, and CE2813 isolates clustered together with a Brazilian H151 isolate. This research reports mixed infections caused by H. capsulatum isolates with different mating types in Brazilian AIDS‑patients for the first time in the literature.
Keywords
Mixed infection
Histoplasma capsulatum
Mating types
Multilocus sequence typing
Histoplasmosis
HIV coinfections
 
Publisher
Universidade de São Paulo
Citation
DAMASCENO, Lisandra Serra et al. Mixed infection by Histoplasma capsulatum isolates with different mating types in Brazilian AIDS-patients. Revista do Instituto de Medicina Tropical de São Paulo, v. 61, p. 1-10, 2019.
DOI
10.1590/s1678-9946201961008
ISSN
0036-4665