| Author | Souza, Marta Cristina O. | |
| Author | Freire, Marcos S. | |
| Author | Schulze, Erica A. | |
| Author | Gaspar, Luciane P. | |
| Author | Castilho, Leda R. | |
| Access date | 2019-03-19T19:09:48Z | |
| Available date | 2019-03-19T19:09:48Z | |
| Document date | 2009 | |
| Citation | SOUZA, Marta Cristina O. et al. Production of yellow fever virus in microcarrier-based Vero cell cultures. Vaccine, Amsterdam, v. 27, n. 46, p. 6420-6423, 2009. | pt_BR |
| ISSN | 1873-2518 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/32164 | |
| Language | eng | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Production of yellow fever virus in microcarrier-based Vero cell cultures | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.vaccine.2009.06.023 | |
| Abstract | In this work, the propagation of the 17DD yellow fever virus in Vero cells grown on Cytodex-1 microcarriers was evaluated. After verifying that upon infection the virus adsorption step could be performed under continuous agitation, experiments were carried out in spinners and sparged lab-scale stirred-tank bioreactor to evaluate the use of a commercial serum-free medium (VP-SFM) and to investigate the effects of multiplicity of infection (MOI) and time of infection (TOI) on virus production. Virus titers as high as 8.4 × 108 pfu/mL were obtained upon infection with MOI of 0.02 and TOI of 3 days, using the serum-free medium in the sparged bioreactor. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. COPPE. Laboratório de Engenharia de Cultura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. COPPE. Laboratório de Engenharia de Cultura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. COPPE. Laboratório de Engenharia de Cultura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Yellow fever virus | pt_BR |
| Subject | Microcarrier-based culture | pt_BR |
| Subject | Vero cells | pt_BR |
| Subject | Serum-free medium | pt_BR |
